Basic properties of a novel ryanodine-sensitive, caffeine-insensitive calcium-induced calcium release mechanism in permeabilised human vascular smooth muscle cells

FEBS Letters
S Lynn, J I Gillespie

Abstract

The efflux of 45Ca2+ from preloaded intracellular stores of saponin-permeabilised human uterine artery smooth muscle cultured cells was used to study the mechanisms underlying Ca2+ release from the sarcoplasmic reticulum (SR). The present paper demonstrates directly a functional Ca2+ release mechanism that is dependent on an increase in free Ca2+ (100 nM-30 microM) and is completely inhibited by 20 microM Ruthenium red. The amount of Ca2+ released at 30 microM free Ca2+ was reduced by approximately 50% compared to the release at 10 microM. This Ca(2+)-induced Ca2+ release (CICR) mechanism was not sensitive to caffeine. Exposure of cells to low free Ca(2+)-containing solutions (10 nM) indicated that a component of the CICR mechanism may be functional at basal free Ca2+ levels of 100 nM. Application of ryanodine (0.1-100 microM) induced 45Ca2+ efflux from the sarcoplasmic reticulum and this release was also inhibited by 20 microM Ruthenium red.

References

May 1, 1991·Pflügers Archiv : European journal of physiology·A Herrmann-FrankG Meissner
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K DanoffC A Ross
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·S Fleischer, M Inui
Aug 1, 1989·The Journal of General Physiology·M Iino
Apr 27, 1988·European Journal of Pharmacology·J P Savineau
Apr 22, 1988·Biochimica Et Biophysica Acta·M MichalakV Shoshan-Barmatz
Apr 15, 1988·Biochemical and Biophysical Research Communications·M IinoM Endo
Mar 1, 1986·The Journal of General Physiology·H Yamamoto, C van Breemen
Aug 1, 1984·The Journal of General Physiology·K Saida, C Van Breemen
Jul 1, 1983·The American Journal of Physiology·A Fabiato
Aug 1, 1982·The Journal of General Physiology·K Saida
Feb 1, 1995·Pflügers Archiv : European journal of physiology·J A NichollsJ I Gillespie
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·R M TribeM P Blaustein
Jun 1, 1994·The American Journal of Physiology·R CoronadoD M Vaughan
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·L XuG Meissner
Aug 20, 1993·Biochimica Et Biophysica Acta·J M MorganJ R Greenwell
Jan 28, 1993·Nature·M J Berridge

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Citations

Jul 28, 1999·European Journal of Pharmacology·D M BurnsD G Hirst
May 7, 2010·American Journal of Physiology. Cell Physiology·Thirumalini VaithianathanAlejandro M Dopico
May 14, 2016·Vascular Pharmacology·Daniela C G GarciaSteyner F Côrtes
Dec 17, 2004·Pharmacological Reviews·Régent LaporteIsmail Laher
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Deepak A DeshpandeMathur S Kannan
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P L LiW B Campbell
May 15, 2003·The Journal of Biological Chemistry·Nael Nadif KasriHumbert De Smedt
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Wang-Xian TangPin-Lan Li

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