Basic Properties of the p38 Signaling Pathway in Response to Hyperosmotic Shock

PloS One
Nabil Ben MessaoudJosé M López

Abstract

Some properties of signaling systems, like ultrasensitivity, hysteresis (a form of biochemical memory), and all-or-none responses at a single cell level, are important to understand the regulation of irreversible processes. Xenopus oocytes are a suitable cell model to study these properties. The p38 MAPK (mitogen-activated protein kinase) pathway is activated by different stress stimuli, including osmostress, and regulates multiple biological processes, from immune response to cell cycle. Recently, we have reported that activation of p38 and JNK regulate osmostress-induced apoptosis in Xenopus oocytes and that sustained activation of p38 accelerates cytochrome c release and caspase-3 activation. However, the signaling properties of p38 in response to hyperosmotic shock have not been studied. Here we show, using Xenopus oocytes as a cell model, that hyperosmotic shock activates the p38 signaling pathway with an ultrasensitive and bimodal response in a time-dependent manner, and with low hysteresis. At a single cell level, p38 activation is not well correlated with cytochrome c release 2 h after hyperosmotic shock, but a good correlation is observed at 4 h after treatment. Interestingly, cytochrome c microinjection induces p38 ph...Continue Reading

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Citations

Apr 2, 2020·International Journal of Molecular Sciences·Jicheng Yue, José M López
Oct 30, 2015·The Journal of Biological Chemistry·Jicheng YueJosé M López
Jun 28, 2018·Frontiers in Molecular Neuroscience·Yu-Feng Wang, Vladimir Parpura

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Methods Mentioned

BETA
Assay
nuclear translocation

Software Mentioned

GraphPad Prism4
SAS
GraphPad Prism

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