Basic techniques in mammalian cell tissue culture

Current Protocols in Cell Biology
Katy Phelan, Kristin M May

Abstract

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.

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Feb 22, 2017·International Journal of Molecular Sciences·Allison A AtnipM Mónica Giusti
Mar 4, 2017·Current Protocols in Cell Biology·Xin-Hua Liao, Alan R Kimmel
Sep 15, 2017·Current Protocols in Chemical Biology·Shaozhe YangPingnan Sun
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