Basic vapor exposure for tuning the charge state distribution of proteins in negative electrospray ionization: elucidation of mechanisms by fluorescence spectroscopy.

Journal of the American Society for Mass Spectrometry
Marion GirodGeorge Stafford

Abstract

Manipulation for simplifying or increasing the observed charge state distributions of proteins can be highly desirable in mass spectrometry experiments. In the present work, we implemented a vapor introduction technique to an Agilent Jet Stream ESI (Agilent Technologies, Santa Clara, CA, USA) source. An apparatus was designed to allow for the enrichment of the nitrogen sheath gas with basic vapors. An optical setup, using laser-induced fluorescence and a pH-chromic dye, permits the pH profiling of the droplets as they evaporate in the electrospray plume. Mechanisms of pH droplet modification and its effect on the protein charging phenomenon are elucidated. An important finding is that the enrichment with basic vapors of the nitrogen sheath gas, which surrounds the nebulizer spray, leads to an increase in the spray current. This is attributed to an increase in the electrical conductivity of water-amine enriched solvent at the tip exit. Here, the increased current results in a generation of additional electrolytically produced OH(-) ions and a corresponding increase in the pH at the tip exit. Along the electrospray plume, the pH of the droplets increases due to both droplet evaporation and exposure to basic vapors from the seeded...Continue Reading

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Citations

Oct 6, 2015·Analytical Chemistry·Dmytro S KulykAbraham K Badu-Tawiah
Jun 26, 2015·Analytical Chemistry·Antonin SoleilhacRodolphe Antoine
Apr 4, 2017·Analytical Chemistry·Anneli Kruve, Karl Kaupmees
May 11, 2017·Analytical Chemistry·Piia LiigandAnneli Kruve
Jan 15, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Tristan DoussineauBernadette Charleux
Jan 1, 2015·Journal of Mass Spectrometry : JMS·Christine M FisherScott A McLuckey
May 20, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Cindy ColombéLucie Sancey
Jul 13, 2017·Journal of the American Society for Mass Spectrometry·Feifei ZhaoScott A McLuckey
Jul 27, 2017·Journal of the American Society for Mass Spectrometry·Piia LiigandAnneli Kruve
Dec 7, 2013·Analytical Chemistry·Lars KonermannModupeola A Sowole

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