Basics of flow cytometry-based sterility testing of platelet concentrates

Transfusion
Harald MohrT H Müller

Abstract

Flow cytometry (FACS) is a common technique in blood banking. It is used, for example, for the enumeration of residual white blood cells in plasma and in cellular blood products. It was investigated whether it can also be applied for sterility testing of buffy coat-derived platelet concentrates (PCs). Plasma-reduced PCs were spiked with bacteria and stored at 20 to 24 or 37 degrees C for various times. The following 10 species were used: Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, and Yersinia enterocolitica. Bacterial DNA was stained with thiazole orange. After the platelets were lysed, bacteria were enumerated by FACS. All bacteria species used were detectable by FACS. The lower detection limit was approximately 100 bacteria per microL, that is, 10(5) per mL. In general, the titers measured were 1.2- to 3-fold higher than those determined by colony forming assay. In one case (K. pneumoniae) in which the dot plot of the bacteria cloud overlapped with that of bacteria debris, they were consistently lower. When PC samples were inoculated with approximately 1 colony-forming u...Continue Reading

References

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Citations

Dec 17, 2009·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·T Montag-LessingChristian K Schneider
Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·M SchmidtE Seifried
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·T H MüllerT Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Oliver KaroT Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Mariam Klouche, Uwe Schröder
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·T Montag
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Sep 15, 2006·Proteomics·Pierre-Alain QuelozJean-Daniel Tissot
Sep 27, 2006·Transfusion Medicine·M SchmidtE Seifried
Dec 22, 2006·Vox Sanguinis·M SchmidtG Walther-Wenke
Sep 8, 2011·Transfusion Medicine·G AlbertoniJ A Barreto

Related Concepts

Bacteriological Techniques
Blood Banks
Blood Platelets
Drug Impurity
Flow Cytometry
Platelet Transfusion

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