Basics of flow cytometry-based sterility testing of platelet concentrates

Harald MohrT H Müller


Flow cytometry (FACS) is a common technique in blood banking. It is used, for example, for the enumeration of residual white blood cells in plasma and in cellular blood products. It was investigated whether it can also be applied for sterility testing of buffy coat-derived platelet concentrates (PCs). Plasma-reduced PCs were spiked with bacteria and stored at 20 to 24 or 37 degrees C for various times. The following 10 species were used: Bacillus cereus, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Serratia marcescens, and Yersinia enterocolitica. Bacterial DNA was stained with thiazole orange. After the platelets were lysed, bacteria were enumerated by FACS. All bacteria species used were detectable by FACS. The lower detection limit was approximately 100 bacteria per microL, that is, 10(5) per mL. In general, the titers measured were 1.2- to 3-fold higher than those determined by colony forming assay. In one case (K. pneumoniae) in which the dot plot of the bacteria cloud overlapped with that of bacteria debris, they were consistently lower. When PC samples were inoculated with approximately 1 colony-forming u...Continue Reading


Jul 1, 1990·Journal of Clinical Microbiology·T C ThorpeL B Reller
Apr 28, 1999·Transfusion Medicine Reviews·K M Mitchell, M E Brecher
Jun 6, 2000·Vox Sanguinis·H GullikssonA Shanwell
Oct 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·J R DelangheL Beier
Aug 9, 2001·Transfusion·Roslyn YomtovianViral Activation Transfusion Study Group
Oct 31, 2001·Seminars in Hematology·R J Benjamin
Nov 28, 2001·Hematology·E Snyder, R Y Dodd
Feb 15, 2002·British Journal of Haematology·P F van der MeerJ de Wildt-Eggen
May 8, 2004·Journal of Clinical Microbiology·S RibaultI Besson Faure
Jun 24, 2004·Vox Sanguinis·P R Foster


Dec 17, 2009·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·T Montag-LessingChristian K Schneider
Oct 22, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·M SchmidtE Seifried
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·T H MüllerT Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Oliver KaroT Montag
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Mariam Klouche, Uwe Schröder
Jul 16, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·T Montag
Dec 5, 2006·Expert Review of Molecular Diagnostics·Tamimount MohammadiHenk W Reesink
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker
Sep 15, 2006·Proteomics·Pierre-Alain QuelozJean-Daniel Tissot
Sep 27, 2006·Transfusion Medicine·M SchmidtE Seifried
Dec 22, 2006·Vox Sanguinis·M SchmidtG Walther-Wenke
Sep 8, 2011·Transfusion Medicine·G AlbertoniJ A Barreto

Related Concepts

Bacteriological Techniques
Blood Banks
Blood Platelets
Drug Impurity
Flow Cytometry
Platelet Transfusion

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.