PMID: 8584416Nov 1, 1995Paper

Basolateral membrane chloride permeability of A6 cells: implication in cell volume regulation

Pflügers Archiv : European journal of physiology
E BrochieroJ Ehrenfeld

Abstract

The permeability to Cl- of the basolateral membrane (blm) was investigated in renal (A6) epithelial cells, assessing their role in transepithelial ion transport under steady-state conditions (isoosmotic) and following a hypoosmotic shock (i.e. in a regulatory volume decrease, RVD). Three different complementary studies were made by measuring: (1) the Cl- transport rates (delta F/Fo s-1 (x10(-3))), where F is the fluorescence of N-(6-methoxyquinoyl) acetoethyl ester, MQAE, and Fo the maximal fluorescence (x10(-3)) of both membranes by following the intracellular Cl- activities (ai Cl-, measured with MQAE) after extracellular Cl- substitution (2) the blm 86Rb and 36Cl uptakes and (3) the cellular potential and Cl- current using the whole-cell patch-clamp technique to differentiate between the different Cl- transport mechanisms. The permeability of the blm to Cl- was found to be much greater than that of the apical membranes under resting conditions: aiCl- changes were 5.3 +/- 0.7 mM and 25.5 +/- 1.05 mM (n = 79) when Cl- was substituted by NO3(-) in the media bathing apical and basolateral membranes. The Cl- transport rate of the blm was blocked by bumetanide (100 microM) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 50 ...Continue Reading

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Citations

Jul 6, 2004·Pflügers Archiv : European journal of physiology·L GuerraV Casavola
Mar 7, 2000·Toxicology and Applied Pharmacology·B Faurskov, H F Bjerregaard
Aug 24, 1999·The Journal of Physiology·U BanderaliJ Ehrenfeld
Nov 19, 2014·Evidence-based Child Health : a Cochrane Review Journal·Olabisi OduwoleEkong E Udoh
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Oct 8, 1997·The American Journal of Physiology·C DurantonP Poujeol

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