Basolateral sorting of the coxsackie and adenovirus receptor through interaction of a canonical YXXPhi motif with the clathrin adaptors AP-1A and AP-1B.

Proceedings of the National Academy of Sciences of the United States of America
Jose Maria Carvajal-GonzalezEnrique Rodriguez-Boulan

Abstract

The coxsackie and adenovirus receptor (CAR) plays key roles in epithelial barrier function at the tight junction, a localization guided in part by a tyrosine-based basolateral sorting signal, (318)YNQV(321). Sorting motifs of this type are known to route surface receptors into clathrin-mediated endocytosis through interaction with the medium subunit (μ2) of the clathrin adaptor AP-2, but how they guide new and recycling membrane proteins basolaterally is unknown. Here, we show that YNQV functions as a canonical YxxΦ motif, with both Y318 and V321 required for the correct basolateral localization and biosynthetic sorting of CAR, and for interaction with a highly conserved pocket in the medium subunits (μ1A and μ1B) of the clathrin adaptors AP-1A and AP-1B. Knock-down experiments demonstrate that AP-1A plays a role in the biosynthetic sorting of CAR, complementary to the role of AP-1B in basolateral recycling of this receptor. Our study illustrates how two clathrin adaptors direct basolateral trafficking of a plasma membrane protein through interaction with a canonical YxxΦ motif.

References

Oct 1, 1994·Molecular Biology of the Cell·A K RajasekaranE Rodriguez-Boulan
Nov 15, 1996·The Journal of Biological Chemistry·H OhnoJ S Bonifacino
Feb 14, 1997·The Journal of Biological Chemistry·A H Le GallE Rodriguez-Boulan
Feb 20, 1999·The Journal of Biological Chemistry·D ZizioliP Schu
Jun 3, 1999·The Journal of Cell Biology·J S Bonifacino, E C Dell'Angelica
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Noriyuki NishimuraWilliam E Balch
Jul 10, 2002·Nature Cell Biology·Yunbo GanEnrique Rodriguez-Boulan
Sep 7, 2002·Science·Balraj DorayStuart Kornfeld
Jan 17, 2003·Molecular Biology of the Cell·Satoshi WaguriBernard Hoflack
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jan 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Diego GravottaEnrique Rodriguez-Boulan
Mar 25, 2008·Current Topics in Microbiology and Immunology·P FreimuthS D Carson
Apr 11, 2008·Nature·Sylvie DebordeEnrique Rodriguez-Boulan
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fernando DiazEnrique Rodriguez-Boulan
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Linton M Traub
Oct 27, 2009·FEBS Letters·Alfonso Gonzalez, Enrique Rodriguez-Boulan
Nov 20, 2009·Journal of Cell Science·Ora A Weisz, Enrique Rodriguez-Boulan
Jun 10, 2010·Kidney International·Ryan SchreinerEnrique Rodriguez-Boulan

❮ Previous
Next ❯

Citations

Dec 1, 2012·Nature Cell Biology·Gerard ApodacaDavid M Bryant
Apr 14, 2012·Nature Reviews. Molecular Cell Biology·Victor W HsuJian Li
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bhuminder SinghRobert J Coffey
Jun 30, 2012·American Journal of Physiology. Cell Physiology·Ensaf Y AlmomaniEmmanuelle Cordat
Oct 6, 2012·Journal of Cell Science·Jin XuAparna Lakkaraju
Mar 22, 2014·Nature Reviews. Molecular Cell Biology·Enrique Rodriguez-Boulan, Ian G Macara
Nov 13, 2013·Annual Review of Physiology·Bhuminder Singh, Robert J Coffey
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Roland ThuenauerChing-Hwa Sung
Aug 26, 2014·Annual Review of Cell and Developmental Biology·Yusong GuoRandy Schekman
Feb 14, 2013·The Journal of Biological Chemistry·Gonzalo A MardonesJuan S Bonifacino
Jan 8, 2014·The Journal of Cell Biology·Juan S Bonifacino
Apr 16, 2016·Antiviral Research·Xiang LiWei Yang
Mar 6, 2015·Molecular Biology of the Cell·Erwin de la Fuente-OrtegaEnrique Rodríguez-Boulan
Apr 18, 2013·PloS One·Rachel Clemens GrishamTeresa Nicolson
Dec 23, 2016·Cold Spring Harbor Perspectives in Biology·Paulo S CaceresEnrique J Rodriguez-Boulan
Jan 4, 2017·Virology·Priyanka SharmaKatherine J D A Excoffon
Jan 10, 2013·Archives of Insect Biochemistry and Physiology·Yan-shan NiuYun-gen Miao
Oct 15, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Giuseppina GarianoDaniela Zizioli
Mar 22, 2014·Journal of the American Society of Nephrology : JASN·Emily H Stoops, Michael J Caplan
May 16, 2019·Molecular Biology of the Cell·Diego GravottaEnrique Rodriguez-Boulan
Jul 10, 2019·Annual Review of Virology·Urs F Greber, Justin W Flatt
Apr 17, 2020·FEBS Letters·Katherine J D A Excoffon
May 19, 2016·The FEBS Journal·Stine C KlingerMorten S Nielsen
Feb 3, 2016·The Biochemical Journal·Elisabeth DarrouzetThierry Pourcher
Oct 28, 2015·Biomedical Optics Express·James A LevittKlaus Suhling
Aug 28, 2020·Investigative Ophthalmology & Visual Science·Ji Sun LeeJaya Rajaiya
May 31, 2019·Proceedings of the National Academy of Sciences of the United States of America·Paulo S CaceresEnrique Rodriguez-Boulan
Aug 29, 2020·Journal of Cell Science·Tina StormClare E Futter
Mar 25, 2019·Frontiers in Genetics·Wladislaw StroukovRoland Thuenauer
Nov 2, 2019·Advances in Biological Regulation·Stefanie L MakowskiSeth J Field
Jan 18, 2015·Biology Open·Yifan ZhangDhasakumar S Navaratnam
Jun 3, 2021·International Journal of Molecular Sciences·Ivana Strazic GeljicTihana Lenac Rovis
Aug 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sergio Garrido-JimenezJose Maria Carvajal-Gonzalez
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Clara Maria Mateos-QuirosJose Maria Carvajal-Gonzalez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.