PMID: 9168235May 20, 1997Paper

Basolateral sorting of the HIV type 2 and SIV envelope glycoproteins in polarized epithelial cells: role of the cytoplasmic domain

AIDS Research and Human Retroviruses
J M BallR W Compans

Abstract

In polarized epithelial cell lines, enveloped viruses are directionally released by asymmetric viral budding at specific plasma membrane domains. Previous studies have shown that HIV-1 budding and gp160 expression occur on basolateral membranes whereas the release of HIV-1 Gag particles, in the absence of the Env glycoproteins, is nonpolarized. We have examined the directional transport and surface expression of HIV-2 and SIV envelope glycoproteins using vaccinia virus recombinants in Vero C1008 polarized epithelial cells. Analogous to HIV-1 gp160, both HIV-2 and SIV surface glycoproteins were preferentially directed to basolateral membranes. Hence basolateral expression appears to be a common property of the glycoproteins of primate lentiviruses. To explore the role of the cytoplasmic domain in directing the HIV-2 and SIV Env glycoproteins to the basolateral surface, stop codons were introduced to mimic the natural cytoplasmic truncations observed following repeated passage of these viruses in culture. These truncated glycoproteins also were sorted to the basolateral domain, but at a lower efficiency than the full-length protein product. In contrast, when the entire cytoplasmic domain of the SIV Env glycoprotein was deleted, t...Continue Reading

References

Jun 1, 1978·The Journal of Cell Biology·M CereijidoD D Sabatini
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D S MisfeldtD R Pitelka
Aug 1, 1991·Current Opinion in Cell Biology·I S Trowbridge
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J OwensR W Compans
Dec 1, 1990·AIDS Research and Human Retroviruses·M P MoyerH E Gendelman
Jun 1, 1990·AIDS Research and Human Retroviruses·M J MulliganR W Compans
Dec 1, 1989·AIDS Research and Human Retroviruses·A HasegawaM Hayami
Aug 18, 1989·Science·E Rodriguez-Boulan, W J Nelson
Oct 19, 1989·Nature·V M HirschJ I Mullins
Mar 1, 1989·The Journal of Membrane Biology·M SargiacomoE Rodriguez-Boulan
Nov 1, 1989·Journal of Virology·T KodamaR C Desrosiers
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M P LisantiE Rodriguez-Boulan
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L PuddingtonJ K Rose
Aug 23, 1988·Biochemistry·K Simons, G van Meer
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A F LopezM A Vadas
Jul 1, 1986·The American Journal of Physiology·R E SteeleJ S Handler

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Citations

Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
Aug 27, 2004·Médecine sciences : M/S·Eugenia BasyukEdouard Bertrand
Dec 8, 2009·Nature Structural & Molecular Biology·Dorota Piekna-PrzybylskaRobert A Bambara
Sep 11, 1999·Journal of Virology·M J VincentR W Compans

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