Basophil production

The Journal of Clinical Investigation
J A DenburgJ Bienenstock

Abstract

Factors influencing basophil production from the bone marrow of ovalbumin (OA)-sensitized guinea pigs have been examined in vitro. Autologous co-cultures of marrow and spleen cells from OA-immune animals contained significantly higher numbers of basophils after 7 d of liquid culture in the presence of OA, compared with control co-cultures or with marrow cultures alone (P < 0.005). Basophils increased in co-culture as the number of spleen cells added to a fixed number of marrow cells was increased from 0.10 to 2.5 x 10(6)/ml; at each spleen cell concentration, the presence of OA significantly enhanced basophil production in vitro when compared with unstimulated co-cultures. There was no basophil production from spleen cell suspensions cultured in the absence of autologous marrow cells. Conditioned media (CM) prepared from OA-stimulated spleen cells of OA-treated animals (CM-OA) caused a specific stimulation of basophil production from normal guinea pig bone marrow cells in liquid cultures (P < 0.01). Phytohemagglutinin (PHA)- and pokeweed mitogen-stimulated CM (CM-PHA, CM-pokeweed mitogen) nonspecifically enhanced normal basophilopoiesis, causing dose-dependent increases in basophils and histamine in vitro. CM-OA and CM-PHA also...Continue Reading

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Citations

Jan 1, 1982·Journal of Immunological Methods·M DyE Schneider
Apr 6, 2004·Immunology and Allergy Clinics of North America·Judah A Denburg, Paul K Keith
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H MatsudaJ A Denburg
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Aug 1, 1983·The Journal of Pediatrics·R F LemanskeD D Metcalfe
Feb 27, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Hirohisa SaitoKimishige Ishizaka
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Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.