PMID: 6406905Jun 9, 1983Paper

Basophil variants with impaired cromoglycate binding do not respond to an immunological degranulation stimulus

Nature
N MazurekI Pecht

Abstract

Cromoglycate, which inhibits IgE-mediated degranulation of mast cells and a basophilic rat tumour cell line (RBL-2H3) (ref.3), is a drug widely used in the treatment of allergic asthma. We have demonstrated the presence of specific binding sites for that drug on the membranes of basophils and mast cells and more recently, we have succeeded in isolating the cromoglycate-binding protein from the membranes of RBL-2H3 cells. These findings together with the chelation by cromoglycate of alkaline-earth ions in low polarity medium, suggest that the binding site of the drug may either be part, or closely related to the calcium gate. To further investigate this, we have selected variants of the RBL-2H3 cell line that are defective in cromoglycate binding but bind IgE normally. In these variants, which have similar histamine content to the parental cells, IgE-mediated challenge did not lead to Ca2+ influx, degranulation and histamine release. In contrast, these cells were able to release histamine on exposure to the Ca2+ ionophore A23187, indicating that the degranulation mechanism distal to the Ca2+ gating step is unaffected. Taken together, these findings suggest that cromoglycate-binding protein has a role in the transmembrane calcium...Continue Reading

References

Apr 3, 1976·British Medical Journal·J C Foreman, L G Garland
Jan 1, 1979·International Archives of Allergy and Applied Immunology·J C Spitzer
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A McGivneyR P Siraganian
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N MazurekI Pecht
Feb 25, 1980·FEBS Letters·N MazurekI Pecht

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Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·D FranziusR Penner
Aug 12, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Egle Passante, Neil Frankish
Dec 1, 1984·Molecular Immunology·R Sagi-EisenbergI Pecht
Jan 1, 1988·International Journal of Immunopharmacology·A TsicopoulosA Capron
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N MazurekI Pecht
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N MazurekI Pecht
Sep 25, 2009·The Journal of Biological Chemistry·Toshiro NikiMitsuomi Hirashima
Jan 1, 1984·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·D J Triggle
Jan 1, 1984·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·C K Naspitz
Apr 22, 2015·Biomaterials·Sabine SellnerMarkus Rehberg
Apr 9, 2008·American Journal of Physiology. Heart and Circulatory Physiology·David J SantoneThomas F Lindsay
Jul 1, 1989·Current Problems in Pediatrics·D NeddenriepR J Lemen

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