Batch production and growth kinetics of hybridomas.

Cytotechnology
O W Merten

Abstract

Batch cultures of mouse-mouse hybridoma cell lines were carried out and their growth and production kinetics investigated. Three main cell specific production patterns (expressed as pg IgG/cell x hour) were found, which can be used as a classification system for hybridoma cell lines (groups I-III). Cells showing the highest IgG-production at the beginning of the batch culture (during the lag and the onset of the log-phase) were classified as either group I and II. The difference was that cell lines of group II showed a second high cell specific production at the onset of the stationary and death phases. Cell lines of group III had a quite constant production of antibodies during their growth; but IgG secretion completely stopped after the beginning of the stationary phase. The implications of these three production patterns on the design of a production process are discussed.

References

Jan 22, 1986·Journal of Immunological Methods·D VelezJ D Macmillan
Jan 22, 1986·Journal of Immunological Methods·S ReuvenyL Miller
May 10, 1985·Journal of Immunological Methods·M BodeusH Bazin
Feb 25, 1983·Journal of Immunological Methods·S Fazekas de St Groth
May 1, 1986·Biotechnology and Bioengineering·G L AltshulerG Belfort

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Citations

Mar 1, 1990·Cytotechnology·B Griffiths
May 31, 1994·Journal of Biotechnology·M HarigaeH Kataoka
Mar 1, 1998·Cytotechnology·R L DuttonM Moo-Young
Oct 13, 1992·Annals of the New York Academy of Sciences·F J CastilloB C Grant

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