Bavachalcone from Cullen corylifolium induces apoptosis and autophagy in HepG2 cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Hae Seong SongSe Chan Kang

Abstract

Cullen corylifolium is a plant widely used in traditional Chinese medicine for its stomachic, anthelmintic, and diuretic properties. Bavachalcone, which is known as a component of C. corylifolium has been reported to inhibit osteoclast differentiation. However, the anticancer efficacy and mechanism of C. corylifolium and bavachalcone have not been studied. Our aim is to determine whether C. corylifolium has an anticancer effect and to identify the apoptosis and autophagy mechanism of bavachalcone. The anti-proliferative activity of C. corylifolium and bavachalcone was measured with MTT assay. Apoptosis was analyzed using Annexin V and propidium iodide staining. The expression of apoptosis, cell cycle and autophagy related gene was evaluated by western blot. Cell cycle stage was investigated with TaliⓇimage-based cytometer. Autophagic activity was assessed using monodansylcadaverine (MDC) staining. C. corylifolium exhibited potent effect on apoptosis in HepG2 cells. To identify which compound in C. corylifolium is responsible for this effect, we determined the effects of psoralen, psoralidin, bavachalcone, and isobavachalcone on the activity of bid, caspase 3, and PARP. Of all the studied compounds, bavachalcone was the most pot...Continue Reading

Citations

Feb 3, 2021·Journal of Natural Products·Yina XiaoIk-Soo Lee
Nov 22, 2020·Antioxidants·Alexey LomovskyOlga Krestinina
Jun 3, 2021·Cancers·Radka MichalkovaJan Mojzis
Aug 14, 2021·Cell Stress & Chaperones·Elham RazaniDavood Bashash

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