Bavachalcone-induced manganese superoxide dismutase expression through the AMP-activated protein kinase pathway in human endothelial cells

Pharmacology
Yanqi DangJin-Wen Xu

Abstract

Mitochondrial oxidative stress has been suggested as a major etiological factor in cardiovascular diseases. Manganese superoxide dismutase (MnSOD) is an essential antioxidant mitochondrial enzyme. Although polyphenols can induce MnSOD expression, their mechanism of action remains unclear. We examined the effect of bavachalcone, a bioactive compound isolated from Psoralea corylifolia, on MnSOD protein expression and explored whether this effect is mediated through the AMP-activated protein kinase (AMPK) signaling pathway. Our data showed that bavachalcone enhanced the luciferase activity of the MnSOD promoter and increased MnSOD mRNA and protein expressions. Moreover, bavachalcone suppressed the mitochondrial superoxide production in endothelial cells. Conversely, bavachalcone stimulated liver kinase B1 and AMPKα phosphorylation. mRNA interference by using short hairpin RNA (shRNA) of AMPK inhibited bavachalcone-induced MnSOD expression. A-769662, an AMPK activator, also stimulated AMPK activity and increased MnSOD expression. Furthermore, AMPK knockdown by shRNA-AMPK reversed the inhibitory effects of bavachalcone on mitochondrial superoxide production in endothelial cells. These findings indicate that bavachalcone can protect ...Continue Reading

Citations

Feb 24, 2016·Acta Pharmaceutica Sinica. B·David Grahame Hardie
Jul 23, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yanqi DangJin-Wen Xu
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu Jin KimSoo-Jin Jeong
Dec 16, 2017·Phytotherapy Research : PTR·Fiaz AlamMuhammad Hassham Hassan Bin Asad
Oct 6, 2017·Andrology·V Calle-GuisadoL J Garcia-Marin
Jul 14, 2020·Oxidative Medicine and Cellular Longevity·Yi-Ting ZhouJin-Wen Xu
Dec 20, 2018·International Journal of Molecular Sciences·Anastasiya StrembitskaIan P Salt
Dec 14, 2019·Chinese Journal of Natural Medicines·Qi-Hang ZhouGuang-Bo Ge
Aug 17, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kamel CharradiEzzedine Aouani

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