Bax deficiency in mice increases cartilage production during fracture repair through a mechanism involving increased chondrocyte proliferation without changes in apoptosis

Bone
Charles H RundleSubburaman Mohan

Abstract

This study sought to determine the role of the pro-apoptotic gene, Bax, in fracture healing by comparing femoral fracture healing in Bax knockout (KO) and wild-type C57BL/6J (background strain) mice. Bax KO fractures were larger, had more bone mineral content, had approximately 2-fold larger cartilage area per callus area in the first and second weeks of fracture healing, and showed an increased osteoclast surface area in the third and fourth weeks of fracture healing compared to C57BL/6J fractures. The increased cartilage area in the Bax KO fracture callus was due to increases in number of both pre-hypertropic and hypertropic chondrocytes. TUNEL analysis showed no significant differences in the number of either chondrocyte or non-chondrocyte apoptotic cells between Bax KO and C57BL/6J fractures at 7 or 14 days post-fracture, indicating that the increased number of chondrocytes in Bax KO fractures was not due to reduced apoptosis. Analysis of expression of apoptotic genes revealed that although the expression levels of Bcl-2 and Bcl-xL were not different between the Bax KO and C57BL/6J mice at 7 or 14 days post-fracture, the expression of BH3-domain only Bak and "Bik-like" pro-apoptotic gene increased approximately 1.5-fold and...Continue Reading

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Citations

Sep 15, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chikara UshikuDavid W Rowe
Feb 26, 2015·Development, Growth & Differentiation·Kwok Yeung TsangKathryn S E Cheah
Sep 10, 2014·Journal of Biomedical Materials Research. Part a·Li YuJohn P Fisher
Jun 29, 2011·Biochemical and Biophysical Research Communications·Shiyong YangBo Han
Feb 20, 2015·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hirohisa KawahataRyuichi Morishita
Dec 21, 2010·Bone·David E RothemMichael Soudry
Aug 15, 2013·Physiological Genomics·Shaohong ChengSubburaman Mohan

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