Apr 24, 2020

Novel insights into Entamoeba cyst wall formation and the importance of actin cytoskeleton

BioRxiv : the Preprint Server for Biology
Sudip K GhoshS. Nayak


The cyst wall of Entamoeba histolytica, the causative agent of the amoebiasis, is a potential target for new drugs. The "Wattle and Daub" model of cyst wall formation of Entamoeba invadens had already been reported. In this study, we demonstrate in more detail the morphological stages of chitin wall formation in E. invadens using fluorescent chitin-binding dyes and immunolocalization of the cyst wall proteins. Here, the expression and localization of chitin synthase and the importance of actin cytoskeleton dynamics at cellular level, during encystations have been demonstrated for the first time. Chitin deposition was found to be initiated on the cell surface mostly from one distinct point, though multipoint initiation was also observed sometimes. From these points, the wall grew outwards and gradually covered the entire cyst surface with time. The initiation of chitin deposition was guided by the localization of chitin synthase 1 on the plasma membrane. The gradual formation of the cyst wall follows the Wattle and daub model. The chitin deposition occurred on the foundation of Jacob lectin at the cell membrane, and the other cyst wall components, like chitinase, and Jessie were also found to be present in the growing incomplete...Continue Reading

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Mentioned in this Paper

Hematologic Neoplasms
Regulation of Biological Process
Somatic Mutation
Recurrent Malignant Neoplasm
Whole Exome Sequencing
Cancer Treatment
Recurrence (Disease Attribute)

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