Bayesian validation of a serum and milk ELISA for antibodies against Mycobacterium avium subspecies paratuberculosis in Greek dairy goats across lactation

Journal of Dairy Science
E AngelidouL Leontides

Abstract

We validated a commercial (Idexx Pourquier, Montpellier, France) serum and milk indirect ELISA that detects antibodies against Mycobacterium avium ssp. paratuberculosis (MAP) in Greek dairy goats. Each goat was sampled 4 times, starting from kidding and covering early, mid, and late lactation. A total of 1,268 paired milk (or colostrum) and serum samples were collected during the 7-mo lactation period. Bayesian latent class models, which allow for the continuous interpretation of test results, were used to derive the distribution of the serum and milk ELISA response for healthy and MAP-infected individuals at each lactation stage. Both serum and milk ELISA, in all lactation stages, had average and similar overall discriminatory ability as measured by the area under the curve (AUC). For each test, the smallest overlap between the distribution of the healthy and MAP-infected does was in late lactation. At this stage, the AUC was 0.89 (95% credible interval: 0.70; 0.98) and 0.92 (0.74; 0.99) for the milk and serum ELISA, respectively. Both tests had comparable sensitivities and specificities at the recommended cutoffs across lactation. Lowering the cutoffs led to an increase in sensitivity without serious loss in specificity. In c...Continue Reading

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Citations

Aug 11, 2015·Veterinary Microbiology·P A Windsor
Apr 2, 2013·Research in Veterinary Science·Shyamala ThirunavukkarasuRichard J Whittington
May 1, 2018·Journal of Dairy Science·Leslie J Verteramo ChiuYrjo T Grohn
Jun 9, 2019·Comparative Immunology, Microbiology and Infectious Diseases·Manju SinghJagdip Singh Sohal

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