Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells

Biology Open
Jaffer ShahabA Wodarz

Abstract

Apico-basal polarity is the defining characteristic of epithelial cells. In Drosophila, apical membrane identity is established and regulated through interactions between the highly conserved Par complex (Bazooka/Par3, atypical protein kinase C and Par6), and the Crumbs complex (Crumbs, Stardust and PATJ). It has been proposed that Bazooka operates at the top of a genetic hierarchy in the establishment and maintenance of apico-basal polarity. However, there is still ambiguity over the correct sequence of events and cross-talk with other pathways during this process. In this study, we reassess this issue by comparing the phenotypes of the commonly used baz(4) and baz(815-8) alleles with those of the so far uncharacterized baz(XR11) and baz(EH747) null alleles in different Drosophila epithelia. While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones. Further analysis reveals that the chromosomes c...Continue Reading

References

Nov 9, 2001·Annual Review of Genetics·U TepassR Fehon
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D N CoxY N Jan
Sep 27, 2002·Genesis : the Journal of Genetics and Development·Shigeo HayashiSatoshi Goto
Jan 3, 2003·Nature Cell Biology·David BilderNorbert Perrimon
Jan 4, 2003·Nature Cell Biology·Kevin Johnson, Andreas Wodarz
Dec 6, 2003·The Journal of Cell Biology·Melissa M RollsChris Q Doe
Sep 1, 2005·The Journal of Cell Biology·Tony J C Harris, Mark Peifer
Aug 30, 2007·Journal of Cell Science·Scott X AtwoodKenneth E Prehoda
Nov 6, 2007·Developmental Cell·Bob Goldstein, Ian G Macara
Feb 29, 2008·Seminars in Cell & Developmental Biology·Xiaodong WuLaurel A Raftery
Jan 22, 2009·Developmental Cell·Manos MavrakisJennifer Lippincott-Schwartz
May 27, 2009·The Journal of Cell Biology·Melanie A McGillTony J C Harris
Oct 1, 2009·Journal of Cell Science·Soya KimAndreas Wodarz

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Citations

Mar 16, 2019·Journal of Cell Science·Sergio Casas-Tintó, Alberto Ferrús
Apr 4, 2017·Journal of Cell Science·Joseph P CampanaleDenise J Montell
Jul 10, 2018·F1000Research·Yang Hong
Aug 29, 2019·Annual Review of Cell and Developmental Biology·Melissa A PickettJessica L Feldman
Feb 11, 2021·The FEBS Journal·Barry J Thompson
Jul 10, 2021·Cells & Development·Teresa BonelloBarry J Thompson
Sep 3, 2021·Neuropsychiatric Disease and Treatment·Wen-Jie WangZixiao Li

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Methods Mentioned

BETA
dissection
Protein Assay
electrophoresis
X-ray

Software Mentioned

Illustrator CS3
R
Photoshop CS3
MARCM

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