BB rat thymocytes cultured in the presence of islets lose their ability to transfer autoimmune diabetes

Diabetes
B J WhalenA A Rossini

Abstract

Thymocytes from adult BB rats can adoptively transfer autoimmune diabetes to athymic recipients. It is also known that the development of BB rat T-cells is recapitulated in adult thymus organ cultures (ATOCs). Based on these observations, we tested the hypothesis that cells capable of the adoptive transfer of diabetes would be present in long-term ATOCs but could be rendered nondiabetogenic by co-culture with appropriate antigens. We observed that cells recovered from adult diabetes-resistant BB (BBDR) rat thymi cultured for up to 14 days can adoptively transfer disease to athymic WAG-rnu/rnu rats treated with polyinosinic: polycytidylic acid and a monoclonal antibody to preclude development of ART2a+ regulatory T-cells. Co-culture of adult BBDR thymi in the presence of BBDR thyrocytes had no effect on the ability of recovered cells to induce diabetes in 70-80% of adoptive recipients. In contrast, co-culture in the presence of islets prevented transfer of diabetes, on average, in >90% of recipients. Fresh islets, frozen islets, and islets pretreated with streptozotocin to deplete insulin were equally effective in preventing diabetes, but none prevented insulitis in nondiabetic recipients. Co-culture in the presence of islets wa...Continue Reading

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Citations

Dec 18, 2001·Diabetes/metabolism Research and Reviews·B NazlielB Koçer
Mar 29, 2002·Diabetes/metabolism Research and Reviews·Alberto Pugliese, Donatella Miceli
Feb 27, 2003·Journal of Autoimmunity·Barbara J WhalenDale L Greiner
Sep 24, 2013·Molecular BioSystems·Sompop BencharitJohn B Buse
May 13, 2004·Critical Care Nursing Quarterly·Deirdre R Blake, David M Nathan
Jul 24, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D L GreinerJ P Mordes
Oct 29, 2002·Diabetes·John P MordesElizabeth P Blankenhorn

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