PMID: 11920613Mar 29, 2002Paper

bc10: A novel human bladder cancer-associated protein with a conserved genomic structure downregulated in invasive cancer

International Journal of Cancer. Journal International Du Cancer
Irina GromovaJ E Celis

Abstract

To identify novel genes that may be associated with the invasive phenotype of bladder cancer, we compared the mRNA expression profiles of fresh noninvasive (grade II, Ta) and invasive (grade III, T2-T4) human transitional cell carcinomas (TCCs) by mRNA differential display. Using this approach, we isolated a novel gene, designated bc10 (bladder cancer, Mr 10 kDa) that was exclusively expressed in the noninvasive lesions as judged by reverse transcriptase polymerase chain reaction analysis of a panel of 30 grade II, Ta and grade III, T2-T4 TCCs. The full-length bc10 cDNA contains a complete open reading frame (ORF) of 263 bp and encodes a protein composed of 87 amino acids that has no homology to any of the known protein families. Transient expression of bc10 cDNA in COS1 cells yielded a primary translation product with an apparent Mr of 9.8 kDa and pI of 6.7, in agreement with the theoretical calculated value. Comparison of mouse and human bc10 genomic loci revealed an intronless organization of the coding region in both species as well as a highly conserved structure having 91% and 100% identity at the DNA (coding region) and protein levels, respectively. Southern analysis did not reveal gross DNA rearrangements within the bc1...Continue Reading

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Citations

Sep 29, 2009·Molecular & Cellular Proteomics : MCP·José M A MoreiraIrina Gromova
Jun 17, 2010·Briefings in Functional Genomics·David Monk
Feb 26, 2005·Nucleic Acids Research·Erez Y LevanonEli Eisenberg
Oct 3, 2007·Genome Research·Nurit PazGideon Rechavi
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Oct 11, 2012·PloS One·Irina GromovaJosé M A Moreira
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Aug 17, 2011·Experimental Biology and Medicine·De-Gang FanJian-Li Jiang
Mar 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongshuo ZhangBo Fan
Nov 13, 2009·International Journal of Cancer. Journal International Du Cancer·Federica GaleanoAngela Gallo
Dec 1, 2017·PloS One·Irina GromovaJosé M A Moreira
Sep 8, 2005·Circulation·Lianglin ZhangErkki Ruoslahti
May 6, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zehua ZuoXinxing Wu
Sep 13, 2012·Experimental and Therapeutic Medicine·Min PengXinxing Wu
May 3, 2018·Frontiers in Neuroscience·Dessislava MladenovaJohn S Mattick
Feb 7, 2008·Journal of the American Chemical Society·Minkui Luo, Vern L Schramm
Dec 10, 2021·PloS One·Knud Larsen, Mads Peter Heide-Jørgensen

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