BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis.

Scientific Reports
Jing WangKun Yang

Abstract

Non-small-cell lung cancer (NSCLC) represents most of lung cancers, is often diagnosed at an advanced metastatic stage. Therefore, exploring the mechanisms underlying metastasis is key to understanding the development of NSCLC. The expression of B cell receptor-associated protein 31 (BCAP31), calreticulin, glucose-regulated protein 78, and glucose-regulated protein 94 were analyzed using immunohistochemical staining of 360 NSCLC patients. It resulted that the high-level expression of the four proteins, but particularly BCAP31, predicted inferior overall survival. What's more, BCAP31 was closely associated with histological grade and p53 status, which was verified by seven cohorts of NSCLC transcript microarray datasets. Then, three NSCLC cell lines were transfected to observe behavior changes BCAP31 caused, we found the fluctuation of BCAP31 significantly influenced the migration, invasion of NSCLC cells. To identify the pathway utilized by BCAP31, Gene Set Enrichment Analysis was firstly performed, showing Akt/m-TOR/p70S6K pathway was the significant one, which was verified by immunofluorescence, kinase phosphorylation and cellular behavioral observations. Finally, the data of label-free mass spectroscopy implied that BCAP31 p...Continue Reading

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Citations

Jun 26, 2020·Frontiers in Molecular Biosciences·Shuya YangKun Yang
Jan 14, 2021·Molecular Biology Reports·Mwichie NamusambaBing Wang
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Xiyang ZhangKun Yang
Apr 29, 2021·Seminars in Cancer Biology·Chunmei FanYong Li
May 1, 2021·Biochimie·Esben M Quistgaard

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Methods Mentioned

BETA
transfection
transfections
transfectio
GTPase
reverse-transcription PCR
Assay
electrophoresis
confocal microscopy

Software Mentioned

HoloMonitor
MultiCycle
SPSS
Real Time Cellular Analysis ( RTCA )
RTCA
GSEA

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