BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells

Biochemical and Biophysical Research Communications
Myung-Ju OhByung H Jhun

Abstract

BCAR3 (breast cancer anti-estrogen resistance 3) is a signal transducer containing an SH2 domain, a proline/serine-rich domain and a GDP-exchange factor homologous domain, whose role in signaling pathways is currently unclear. Furthermore, BCAR3 is implicated in anti-estrogen resistance of breast cancer cells. In the present study, we investigated the functional role of BCAR3 in a mitogenic signaling pathway of EGF in non-tumorigenic human breast epithelial MCF-12A cells. Microinjection of an anti-BCAR3 antibody, siRNAs targeting BCAR3 and an SH2 domain of BCAR3 inhibited EGF-induced DNA synthesis. Direct association of BCAR3 with activated EGF receptor and Cas was observed. Lastly, microinjection of a BCAR3 expression plasmid induced DNA synthesis. These findings suggest that the BCAR3 protein, through its SH2 domain, is involved in the signaling pathways of EGF leading to cell cycle progression, and that BCAR3 itself is part of a mitogenic signaling pathway.

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Citations

Jun 10, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·P PennekampB Dworniczak
Dec 12, 2012·Genes & Cancer·Yann WallezStefan J Riedl
Mar 11, 2011·Bioscience, Biotechnology, and Biochemistry·Seung-Soo KimCheol-Koo Lee
Nov 13, 2013·Biochemical and Biophysical Research Communications·Myung-Ju OhByung H Jhun
Mar 8, 2018·BioMed Research International·Sun-Ju YiKyunghwan Kim
Dec 20, 2018·Journal of Translational Medicine·Weilong ZhangHongmei Jing
Oct 15, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kiflai BeinGeorge D Leikauf
Jun 26, 2021·ELife·Elizabeth M SteenkisteJonathan A Cooper

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