Bcl-2 expression by CD4 T lymphocytes in Vogt-Koyanagi-Harada disease

Ocular Immunology and Inflammation
Kouichi Ohta, Nagahisa Yoshimura

Abstract

To determine whether Bcl-2 is expressed on CD4(+ ) lymphocytes in the aqueous humor (AH) and cerebrospinal fluid (CSF) of patients with Vogt-Koyanagi-Harada (VKH) disease, and to determine whether Fas will induce apoptosis of lymphocytes in the CSF. The percentages of CD4, CD8, CD45RO, Fas, and Bcl-2 positive T lymphocytes in the AH and CSF of eight patients with active VKH and five healthy controls were determined by flow cytometry. Soluble Fas ligand (sFasL) in the CSF was measured by ELISA. Freshly isolated cells from the CSF were cultured with anti-Fas antibody (Ab) and apoptosis was assessed by the TUNEL method. Fas(+) CD4(+) lymphocytes were the predominant lymphocytes in the AH and CSF of VKH patients. Bcl-2 was strongly expressed in these cells. Soluble FasL was also detected in the CSF. The number of apoptotic cells detected by anti-Fas Ab was not significantly increased in the CSF of VKH patients. In spite of the high expression of Fas antigen on CD4(+) cells and the presence of sFasL in the CSF, apoptosis was not observed. Bcl-2 expression may contribute to the regulation of apoptosis of inflammatory cells in the CSF of VKH patients.

References

Dec 1, 1995·The Journal of Experimental Medicine·N KayagakiH Yagita
Jul 1, 1998·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·K Ohta, N Yoshimura
Sep 27, 2000·The British Journal of Ophthalmology·S SugitaM Mochizuki

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