Bcl-2 expression mediated by Cre/loxP system in olfactory epithelium

Neuroscience Letters
Kiyoshi DoiToshio Terashima

Abstract

To study the Bcl-2 expression mediated by the Cre/loxP recombination system and its effect on prevention of apoptosis in olfactory epithelium. Adenoviral vectors with cassette for Bcl-2 (AxCALNLBcl-2) and Cre recombinase (AxCANCre) were applied to mouse olfactory epithelium by intranasal instillation. The effect of exogenous Bcl-2 expression on prevention of apoptosis of olfactory receptor neurons was investigated using an apoptosis model induced by bulbectomy. The Bcl-2 product was expressed not only in the olfactory receptor neurons but also in the supporting cells. Although statistical analysis did not show significant difference, the number of apoptotic cells in the infected olfactory epithelium on post-bulbectomy day 2 was lower than that of control and the number of survived mature olfactory receptor neurons in the infected olfactory epithelium on post-bulbectomy day 5 was higher than that of control. Although further studies are required for clinical application, the results of our study suggest that this strategy may be able to deliver exogenous Bcl-2 for the treatment of degeneration of olfactory receptor neurons.

References

Sep 1, 1990·Trends in Neurosciences·A I Farbman
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·P G FarlieO Bernard
Aug 26, 1998·Journal of Neurobiology·A L CalofJ Shou
Mar 30, 2002·Chemical Senses·Nanako KinoshitaYoshihiro Yoshihara
Oct 31, 2002·Acta Oto-laryngologica·Yukiko ArimotoHiroshi Shirasawa
Oct 8, 2004·Brain Research. Molecular Brain Research·Kyoko IsoyamaHiroshi Shirasawa

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis