Bcl-2 inhibits apoptosis and extends recombinant protein production in cells infected with Sindbis viral vectors.

Cytotechnology
A J MastrangeloM J Betenbaugh

Abstract

Viruses carrying foreign genes are often used for the production of recombinant proteins in mammalian cells and other eukaryotic expression systems. Though high levels of gene expression are possible using viral vectors, the host cell generally responds to the infection by inducing apoptotic cell death within several days, abruptly ending protein production. It has recently been demonstrated, however, that apoptosis can be suppressed in virally infected cells using anti-apoptotic genes, such as bcl-2. In this study, stably transfected rat carcinomal cell lines, AT3-bcl2 and AT3-neo, were infected with a Sindbis virus carrying the gene for chloramphenicol acetyltransferase (CAT) in an effort to determine the effect of bcl-2 on cell viability and recombinant protein production. Infected AT3-bcl2 cells consistently maintained viabilities close to 100% and a growth rate equivalent to that of uninfected cells (0.040 h(-1)). In contrast, the Sindbis viral vector induced apoptosis in the AT3-neo cells, which were all dead by three days post-infection. Though infected AT3-neo cells generated higher levels of heterologous protein, over 1000 mUnits per well, CAT activity fell to zero by two days post-infection. In contrast, chloramphenic...Continue Reading

References

Oct 8, 1992·Nature·R P BissonnetteD R Green
Jan 1, 1995·Methods in Cell Biology·A J McGahonD R Green
Nov 1, 1995·The Journal of General Virology·I MoriY Kimura
Apr 1, 1995·Current Opinion in Biotechnology·A J Mastrangelo, M J Betenbaugh
Feb 1, 1995·Current Opinion in Genetics & Development·Y Shen, T E Shenk
May 1, 1994·Bio/technology·P J Barr, L D Tomei
Jun 1, 1994·Trends in Biotechnology·A Fanidi, G Evan
Oct 1, 1994·Current Opinion in Biotechnology·P Liljeström
Apr 1, 1995·Trends in Biotechnology·T G Cotter, M al-Rubeai
Jan 1, 1994·Methods in Cell Biology·H Y Naim, M G Roth
Dec 1, 1994·Protein Expression and Purification·T A HsuM J Betenbaugh
Dec 1, 1993·Genes & Development·E White
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Mar 1, 1993·Journal of Neurochemistry·S P MahD E Bredesen
Mar 1, 1993·Immunology Today·J J Cohen
Feb 8, 1996·Nature·E H ChengJ M Hardwick
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·B LevineJ M Hardwick
Jan 1, 1994·Biotechnology Advances·D D Mosser, B Massie
Sep 5, 1994·Biotechnology and Bioengineering·R P SinghA N Emery

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Citations

Jan 15, 2008·Applied Microbiology and Biotechnology·Kelly Astley, Mohamed Al-Rubeai
Nov 13, 2008·Cytotechnology·Nilou Arden, M J Betenbaugh
Mar 6, 1998·Trends in Biotechnology·A J Mastrangelo, M J Betenbaugh
Mar 31, 1999·Trends in Biotechnology·M FusseneggerP P Mueller
May 17, 2000·Journal of Biotechnology·B T TeyM Al-Rubeai
May 20, 1998·Current Opinion in Biotechnology·M al-Rubeai, R P Singh
Jul 23, 2009·Biotechnology and Bioengineering·Kalbinder Singh Sandhu, Mohamed Al-Rubeai
Dec 11, 2002·Biotechnology and Bioengineering·Tina M SauerwaldMichael J Betenbaugh
Feb 10, 1999·Biotechnology and Bioengineering·J GoswamiD I Wang

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