Bcl-2 phosphorylation triggers autophagy switch and reduces mitochondrial damage in limb remote ischemic conditioned rats after ischemic stroke

Translational Stroke Research
Zhifeng QiYumin Luo

Abstract

Autophagy, an important intracellular degradation pathway, has been reported to clear impaired mitochondria and reduce mitochondria-mediated injury in ischemic disease. Our study and other recent investigations have shown that AKT-dependent autophagy contributes to the neuroprotection afforded by limb remote ischemic conditioning (RIC) in experimental stroke. However, how AKT triggers RIC-based autophagy and whether RIC-afforded autophagy is beneficial for mitochondrial function after cerebral ischemia remains unclear. The disruption of the Bcl-2/Beclin1 complex has been reported to trigger autophagy formation in the condition of Bcl-2 phosphorylation at Ser70. We investigated whether Bcl-2 phosphorylation triggers RIC-based autophagy and thereby confers RIC-induced neuroprotection against mitochondrial injury, using a transient cerebral ischemic rat model. We demonstrated that rats undergoing RIC treatment 30 min after the onset of ischemia (I-30) and at reperfusion (R-0) significantly upregulated Bcl-2 phosphorylation. Immunoprecipitation revealed that RIC increased dissociation of the Bcl-2/Beclin1 complex, leading to a higher level of autophagy than in ischemia/reperfusion rats. Furthermore, AKT activation was shown to play...Continue Reading

References

Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
May 24, 2008·Autophagy·Beth LevineGuido Kroemer
Jun 24, 2008·Molecular Cell·Yongjie WeiBeth Levine
Feb 26, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Heng Zhao
Oct 24, 2009·The Journal of Biological Chemistry·Chisato KubotaToshiyuki Takeuchi
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Stephen W G Tait, Douglas R Green
Nov 26, 2010·Experimental Neurology·Daniel J MetcalfDavid C Rubinsztein
Jan 21, 2011·Nature·Beth LevineHerbert W Virgin
Aug 13, 2011·Stroke; a Journal of Cerebral Circulation·Cecil D HahnAndrew N Redington
Jan 26, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jing SunLize Xiong
May 19, 2012·Free Radical Biology & Medicine·María MoránCristina Ugalde
Jul 12, 2012·Nature Biotechnology·Heesun CheongCraig B Thompson
Jul 31, 2012·Free Radical Biology & Medicine·Satish Srinivasan, Narayan G Avadhani
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Jun 14, 2013·Cell & Bioscience·Kai SunLixin Wei
Nov 10, 2013·Stroke; a Journal of Cerebral Circulation·Kristina Dupont HougaardGrethe Andersen
Jan 11, 2014·Progress in Neurobiology·Fan ChenXunming Ji
May 20, 2014·Biochimica Et Biophysica Acta·Yu MeiXiaoYong Tong
Jul 1, 2008·Autophagy·Beth LevineGuido Kroemer

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Citations

Jan 27, 2016·Reviews in the Neurosciences·Jingrui PanYing Peng
Aug 16, 2016·Translational Stroke Research·Eleonora Napoli, Cesar V Borlongan
Jan 14, 2017·Expert Opinion on Therapeutic Targets·Jinghan FengJiangang Shen
Feb 22, 2017·Medical Gas Research·Lei Huang
Dec 15, 2018·Journal of Neuroscience Research·Robert P Ostrowski, John H Zhang
Nov 22, 2016·Medical Gas Research·Jun-Long HuangQin Hu
Oct 3, 2018·Aging and Disease·Fan LiuQin Hu
Jun 2, 2020·Translational Neurodegeneration·Luodan YangQuanguang Zhang
Feb 2, 2021·Frontiers in Cellular Neuroscience·Cedric Bressan, Armen Saghatelyan
Nov 3, 2020·Frontiers in Cellular Neuroscience·Jie LiuYi Yang
Mar 8, 2021·Experimental Neurology·Xiaoyu WangJianmin Zhang
Apr 7, 2021·Pharmacology & Therapeutics·Amir AjoolabadyJun Ren
May 1, 2021·Archives of Medical Research·Nasrin AbolhasanpourLeila Hosseini

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