Bcl-2 sustains hormetic response by inducing Nrf-2 nuclear translocation in L929 mouse fibroblasts

Free Radical Biology & Medicine
Armando Luna-LópezMina Königsberg

Abstract

Hormesis is the process whereby exposure to a low dose of a chemical agent induces an adaptive effect on the cell or organism. This response evokes the expression of cytoprotective and antioxidant proteins, allowing pro-oxidants to emerge as important hormetic agents. The antiapoptotic protein Bcl-2 is known to protect cells against death induced by oxidants; it has been suggested that Bcl-2 might also modulate steady-state reactive oxygen species levels. The aim of this work was to find out if Bcl-2 might play a role during the hormetic response and in Nrf-2 activation. We have established a model to study the oxidative conditioning hormesis response (OCH) by conditioning the cell line L929 with 50muM H(2)O(2) for 9h. This condition did not induce oxidative damage nor oxidative imbalance, and OCH cells maintained a 70-80% survival rate after severe H(2)O(2) treatment compared to nonconditioned cells. When cells were pretreated with the Bcl-2 inhibitor HA14-1 or were silenced with Bcl-2-siRNA, both the hormetic effect and the Nrf-2 nuclear translocation previously observed were abrogated. Our results suggest a sequence of causal events related to increase in Bcl-2 expression, induction of Nrf-2 activation, and sustained express...Continue Reading

Citations

Dec 14, 2011·Cell Biology International·Norma Edith López-DiazguerreroMina Königsberg
Aug 16, 2015·Biochimica Et Biophysica Acta·Andres A CaroThomas E Goodwin
Jan 22, 2013·Biochimie·Denise Clavijo-CornejoLuis E Gómez-Quiroz
Feb 25, 2015·Archives of Dermatological Research·Agnieszka Gęgotek, Elżbieta Skrzydlewska
Oct 7, 2014·Journal of Cell Communication and Signaling·Armando Luna-LópezMina Königsberg
Dec 15, 2019·Archives of Physiology and Biochemistry·Rachid AkkiAngela Marino
Jun 9, 2020·Oxidative Medicine and Cellular Longevity·Pedro Posadas-RodríguezArmando Luna-López
Nov 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Edward J Calabrese, Walter J Kozumbo

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