Bcl-X is the major pleiotropic anti-apoptotic gene activated by retroviral insertion mutagenesis in an IL-3 dependent bone marrow derived cell line

Oncogene
J ThomasJ Marvel

Abstract

In order to identify genes capable of inhibiting apoptosis induced by different pathways, without inducing proliferation we have performed retroviral insertion mutagenesis in the IL-3 dependent bone marrow derived Baf-3 cell line. Out of 200 mutants obtained in three separate mutagenesis experiments, four mutants were resistant to multiple apoptosis inducing pathways (including growth factor starvation, staurosporine, etoposide and cyclosporin A) and did not proliferate in the absence of IL-3. These four mutants overexpress the bcl-X gene following a retroviral insertion 5' of the translation initiation site. These results indicate that the bcl-X gene is a major pleiotropic anti-apoptotic gene in Baf-3 cells. They also suggest that the Bcl-2 family of genes might be the only one capable of inhibiting apoptosis induced by multiple pathways without inducing cell proliferation.

Citations

Oct 24, 2008·Journal of Virology·Marieke BokhovenMary K Collins
May 23, 2006·Oncogene·E DeniaudY Leverrier
Aug 9, 2001·Oncogene·W LowM K Collins
May 24, 2000·The Journal of Biological Chemistry·K AriyoshiT Kitamura
May 6, 1999·European Journal of Immunology·A CiprésP Williamson
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J C Reed

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