BCL-xL-dependent light scattering by apoptotic cells

Biophysical Journal
N N BoustanyN V Thakor

Abstract

We measured the intensity ratio of wide-to-narrow angle scatter, optical scatter image ratio (OSIR), in single cells during apoptosis and after overexpression of the mitochondria-bound antiapoptotic protein BCL-xL. OSIR is sensitive to particle size/shape for objects with wavelength-scale dimensions, and was used as a morphometric measure of cellular response. Three cell variants were treated with staurosporine (STS): nontransfected parental CSM14.1, CSM14.1 stably expressing yellow fluorescent protein (YFP) with diffuse YFP fluorescence, and apoptosis-resistant CSM14.1 stably expressing the fusion protein construct YFP-BCL-xL with YFP fluorescence localized on the mitochondria. After treatment with 1 or 2 muM STS, the measured OSIR decreased monotonically by approximately 25% in the nontransfected and YFP variants, and reached a steady-state value 40-60 min after STS treatment. The decrease in OSIR at the onset of apoptosis preceded phosphatidyl serine exposure by 5 h. In the YFP-BCL-xL cell variant, the initial OSIR was already approximately 24% lower than the initial OSIR in YFP and nontransfected cells, and only decreased by <10% after STS treatment. Alterations in light scattering by cells overexpressing BCL-xL even before...Continue Reading

References

Jun 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S ReipertT D Allen
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y T HsuR J Youle
Apr 2, 1998·Experimental Cell Research·S Camilleri-BroëtD Hockenbery
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M JürgensmeierJ C Reed
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M NaritaY Tsujimoto
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Jan 10, 2002·Developmental Cell·Luca ScorranoStanley J Korsmeyer
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin OttSten Orrenius
Sep 19, 2002·The Journal of Biological Chemistry·Alicia J KowaltowskiGary Fiskum

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Citations

Jul 14, 2010·Annual Review of Biomedical Engineering·Nada N BoustanyVadim Backman
Sep 17, 2009·Lasers in Medical Science·Freek J van der MeerTon G van Leeuwen
Aug 31, 2010·Journal of Biomedical Optics·Robert M PasternackNada N Boustany
Mar 23, 2007·The Plant Journal : for Cell and Molecular Biology·Martine BoccaraAlbert-Claude Boccara
Jan 18, 2005·Biophysical Journal·Jeremy D WilsonThomas H Foster
Nov 4, 2016·Annals of Biomedical Engineering·Mohammad NaserNada N Boustany
Sep 27, 2018·Journal of Biomedical Optics·Mohammad NaserNada N Boustany
Jun 23, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kevin J ChalutKam W Leong
Mar 9, 2007·Journal of Biomedical Optics·Jeremy D WilsonThomas H Foster
Dec 10, 2009·Optics Express·Jing-Yi ZhengNada N Boustany
Jan 25, 2011·Biomedical Optics Express·Robert M PasternackNada N Boustany
Oct 24, 2019·Molecular Plant-microbe Interactions : MPMI·Natalia Moroz, Kiwamu Tanaka
Feb 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Verena KeitelRalf Kubitz
Jan 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Robert M PasternackNada N Boustany
Aug 4, 2006·Biomaterials·Josephine AllenGuillermo A Ameer
Sep 4, 2007·Biophysical Journal·Jing-Yi ZhengNada N Boustany
Sep 3, 2021·The Review of Scientific Instruments·Ashley E CannadayJames Hoelle

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