PMID: 9539746May 16, 1998Paper

Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation

Proceedings of the National Academy of Sciences of the United States of America
Y HuG Núñez

Abstract

Recent studies indicate that Caenorhabditis elegans CED-4 interacts with and promotes the activation of the death protease CED-3, and that this activation is inhibited by CED-9. Here we show that a mammalian homolog of CED-4, Apaf-1, can associate with several death proteases, including caspase-4, caspase-8, caspase-9, and nematode CED-3 in mammalian cells. The interaction with caspase-9 was mediated by the N-terminal CED-4-like domain of Apaf-1. Expression of Apaf-1 enhanced the killing activity of caspase-9 that required the CED-4-like domain of Apaf-1. Furthermore, Apaf-1 promoted the processing and activation of caspase-9 in vivo. Bcl-XL, an antiapoptotic member of the Bcl-2 family, was shown to physically interact with Apaf-1 and caspase-9 in mammalian cells. The association of Apaf-1 with Bcl-XL was mediated through both its CED-4-like domain and the C-terminal domain containing WD-40 repeats. Expression of Bcl-XL inhibited the association of Apaf-1 with caspase-9 in mammalian cells. Significantly, recombinant Bcl-XL purified from Escherichia coli or insect cells inhibited Apaf-1-dependent processing of caspase-9. Furthermore, Bcl-XL failed to inhibit caspase-9 processing mediated by a constitutively active Apaf-1 mutant,...Continue Reading

References

Oct 1, 1992·Molecular and Cellular Biology·B RubinfeldP Polakis
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E White, R Cipriani
Aug 1, 1994·Current Opinion in Genetics & Development·M O Hengartner, H R Horvitz
Jan 1, 1996·Genes & Development·E White
Jan 2, 1997·Nature·H Duan, V M Dixit
Feb 21, 1997·Science·P Golstein
May 1, 1997·Trends in Biochemical Sciences·K HofmannJ Tschopp
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·S KharbandaD Kufe
Jul 1, 1997·Current Biology : CB·S Seshagiri, L K Miller
Aug 21, 1997·Nature·A M ChinnaiyanV M Dixit
Dec 31, 1997·The Journal of Biological Chemistry·D ChauhanK Anderson
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·N InoharaG Núñez

❮ Previous
Next ❯

Citations

May 17, 2000·Cancer·R N CoffeyJ M Fitzpatrick
Jan 7, 2000·Journal of Cellular Physiology·M S Sheikh, A J Fornace
Apr 11, 2001·Head & Neck·B R Gastman
Jul 12, 2002·Hematological Oncology·Belinda C Baliga, Sharad Kumar
Dec 10, 2002·International Journal of Cancer. Journal International Du Cancer·Weng-Lang YangT S Ravikumar
Aug 16, 2001·International Journal of Hematology·Jane E Parker, Ghulam J Mufti
Oct 24, 2009·Apoptosis : an International Journal on Programmed Cell Death·Ramil CodinaRonald Jemmerson
Jan 15, 2013·Molecular Neurobiology·Ryan S AndertonSherif Boulos
May 4, 2004·Molecular and Cellular Neurosciences·Alexandra KretzStefan Isenmann
Jun 18, 2005·Neuroscience Letters·Sandra EbertRoland Nau
Nov 24, 1999·The American Journal of Medicine·P SaikumarM A Venkatachalam
Sep 29, 2000·Biochemical Pharmacology·M Sioud, M Leirdal
Dec 20, 2000·Brain Research·D W Walke, J I Morgan
Jun 28, 2003·The Journal of Thoracic and Cardiovascular Surgery·James M HammelJeffrey L Segar
Jun 1, 2000·Advances in Enzyme Regulation·E G Krebs, J D Graves
Sep 26, 2003·Journal of Inorganic Biochemistry·John SavoryOthman Ghribi
Aug 14, 1999·Brain Research. Developmental Brain Research·K UraseN Motoyama
Nov 20, 1998·Trends in Genetics : TIG·M M MetzsteinH R Horvitz
Jul 18, 2001·Brain Research. Molecular Brain Research·S SoedaH Shimeno
Mar 27, 2003·Progress in Neuro-psychopharmacology & Biological Psychiatry·Andréa C LeBlanc
May 23, 2002·Biochimie·Kelvin CainGerald M Cohen
May 23, 2002·Biochimie·P A ParoneJ C Martinou
Jun 17, 2000·Progress in Neurobiology·K J BanasiakG G Haddad
Oct 10, 2001·Biochimica Et Biophysica Acta·M ZörnigG Evan
Sep 16, 1999·Mutation Research·E Bossy-Wetzel, D R Green
Oct 31, 1998·Current Opinion in Immunology·M E Peter, P H Krammer
Dec 11, 2002·Current Opinion in Cell Biology·Jerry M Adams, Suzanne Cory
Nov 13, 1998·Current Opinion in Neurobiology·G Nuñez, L del Peso
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Sep 19, 2003·Neurobiology of Disease·Karen L O'MalleyWilliam Holtz
Jun 28, 2003·Neurobiology of Disease·J H WeishauptM Bähr
Apr 20, 2004·Critical Reviews in Oncology/hematology·Jan Maxwell NørgaardPeter Hokland
Oct 18, 2003·Seminars in Immunology·Ignacio Moreno de AlboránCarlos Martínez-A

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.