BCL2 protein expression parallels its mRNA level in normal and malignant B cells.

Blood
Yulei ShenWing C Chan

Abstract

The regulation of B-cell lymphoma 2 (BCL2) protein expression in germinal center (GC) B cells has been controversial. Previous reports have indicated posttranscriptional regulation plays a dominant role. However, a number of recent studies contradicted these reports. Using real-time polymerase chain reaction (PCR) and Standardized Reverse Transcriptase-PCR (StaRT-PCR), we measured the level of mRNA expression in GC, mantle zone (MNZ), and marginal zone (MGZ) cells from laser capture microdissection. Both quantitative RT-PCR measurements of microdissected GC cells from tonsils showed that GC cells had low expression of BCL2 transcripts commensurate with the low protein expression level. These results are in agreement with microarray studies on fluorescence-activated cell sorter (FACS)-sorted cells and microdissected GC cells. We also examined BCL2 mRNA and protein expression on a series of 30 cases of diffuse large B-cell lymphoma (DLBCL) and found, in general, a good correlation. The results suggested that BCL2 protein expression is regulated at the transcriptional level in normal B cells and in the neoplastic cells in most B-cell lymphoproliferative disorders.

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Citations

Jun 21, 2011·Genetic Vaccines and Therapy·Mohammad Feraz Ahsan, Milind M Gore
Feb 19, 2014·The Journal of Experimental Medicine·James Q WangChristopher C Goodnow
Jul 2, 2003·Seminars in Arthritis and Rheumatism·Duane R Schultz, William J Harrington
Jan 19, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Javeed IqbalWing C Chan
Apr 11, 2018·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Meysam AmidfarJoão Quevedo
Dec 2, 2010·Biochemical Society Transactions·Shaun Willimott, Simon D Wagner
Dec 14, 2018·Frontiers in Oncology·Anne Slomp, Victor Peperzak
Jul 25, 2019·Journal of Clinical Medicine·Julie DevinCaroline Bret
Apr 25, 2013·International Journal of Oncology·Berlinda VerdoodtAlireza Mirmohammadsadegh

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