PMID: 15332326Aug 31, 2004Paper

BCNU down-regulates anti-apoptotic proteins bcl-xL and Bcl-2 in association with cell death in oligodendroglioma-derived cells

Journal of Neuro-oncology
Richard A LytleKeith M Rich

Abstract

Oligodendroglial differentiation in gliomas is associated with enhanced sensitivity to chemotherapy. Antiapoptotic proteins, Bcl-xL and Bcl-2, are over-expressed in early passage cell lines derived from glioblastomas. Down-regulation of Bcl-xL and Bcl-2 with DNA antisense oligonucleotides promotes cell death in glioblastoma cells. Changes of expression of Bcl-xL and Bcl-2 after chemotherapy treatment have not been studied in glioma subtypes. The current experiments correlate decreased expression of both Bcl-xL and Bcl-2 after BCNU chemotherapy and cell death in two oligodendroglioma-derived cell lines. Expression of Bcl-2 family member proteins Bcl-xL, Bcl-2, and Bax were assessed in glioma cells both before and after chemotherapy treatment. Cell survival was assessed with a crystal violet bioassay. Levels of expression of Bcl-2 and Bcl-xL were elevated in two early passage oligodendroglioma-derived cell lines compared with a non-neoplastic glial cell line. BCNU chemotherapy markedly down-regulated expression of Bcl-xL and Bcl-2 proteins in both oligodendroglioma-derived cell lines. Changes in expression of Bcl-xL and Bcl-2 were associated with the increased sensitivity to chemotherapy. There were no changes noted in expression...Continue Reading

Citations

Apr 24, 2015·International Journal of Cancer. Journal International Du Cancer·Massimo NabissiGiorgio Santoni
Aug 30, 2008·Nature Medicine·Kimi HonmaTakahiro Ochiya
Oct 29, 2009·Journal of Neurochemistry·Long Tai ZhengKyoungho Suk
Nov 15, 2007·Stem Cells and Development·Mi-Kyung Kang, Soo-Kyung Kang
Jun 24, 2014·Platelets·Jie ZhangKesheng Dai
Sep 20, 2005·Molecular Cancer Therapeutics·Liya YuanKeith M Rich
Aug 23, 2005·Archives of Biochemistry and Biophysics·Maria Pia RigobelloAlberto Bindoli

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