DOI: 10.1101/510743Jan 3, 2019Paper

BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?

BioRxiv : the Preprint Server for Biology
R Jiménez-SaizM Jordana

Abstract

Immunoglobulin (Ig) E is the critical effector molecule in allergic reactions. Consequently, research efforts to understand the biology of IgE-expressing cells is of paramount importance. In particular, the role of IgE+ memory B cells (MBCs) in the perpetuation of allergic reactivity has been the subject of intense research. Studies in mice have convincingly established that IgE+ B cells are rare and transient and, therefore, an unlikely candidate to maintain allergic disease. In contrast, IgE+ MBCs have been detected by flow cytometry in the sputum and peripheral blood of humans and have been proposed as a clinical marker of allergic disease. We established a method to genetically validate, at the single-cell level, the putative IgE+ MBCs identified by flow cytometry from humans. We, then used this information to develop an enhanced flow cytometry protocol that more accurately identifies bona fide IgE+ MBCs. We found that IgE+ MBCs were detected in some patients with atopic dermatitis, but at a frequency that was ~100 times lower than previously reported. We also found that IgE+ MBCs were undetectable in PBMCs from peanut allergic patients. These findings provide tools to identify bona fide IgE+ MBCs, demonstrate their extreme...Continue Reading

Related Concepts

B-Lymphocytes
Biological Markers
Blood Circulation
Dermatitis, Atopic
Flow Cytometry
Food Allergy
Hypersensitivity
Immunoglobulin G
Immunoglobulins
Laboratory mice

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