Bdellovibrio bacteriovorus attacks Aggregatibacter actinomycetemcomitans

Journal of Dental Research
Mark Van EsscheWim Teughels

Abstract

Periodontitis is a polymicrobial infectious disease primarily associated with Gram-negative periodontopathogens. Bdellovibrio and like organisms are predatory bacteria that feed on Gram-negative bacteria. This study investigated whether predatory bacteria can attack Aggregatibacter actinomycetemcomitans. Therefore, A. actinomycetemcomitans was challenged with the predator Bdellovibrio bacteriovorus under conditions simulating the oral cavity. The reduction of planktonic A. actinomycetemcomitans was quantified via bacterial culture, and the development of predatory bacteria was monitored with quantitative real-time PCR. The destruction of A. actinomycetemcomitans biofilms by B. bacteriovorus was quantified by crystal violet staining and visualized by scanning electron microscopy. The in vitro results show that B. bacteriovorus can attack, prey on, and kill A. actinomycetemcomitans and suggest a potential for B. bacteriovorus as a living antibiotic for the prevention and treatment of periodontitis.

References

Nov 1, 1977·Applied and Environmental Microbiology·J M Westergaard, T T Kramer
Feb 1, 1989·Journal of Clinical Periodontology·A J van WinkelhoffJ de Graaff
Jan 1, 1972·Advances in Microbial Physiology·M P STARR, J C Huang
Apr 1, 1982·Infection and Immunity·R J Gibbons, I Etherden
Dec 1, 1993·Oral Microbiology and Immunology·H P MüllerR F Müller
May 21, 1999·Science·J W CostertonE P Greenberg
Jun 23, 2000·Annals of Periodontology·M S Tonetti, A Mombelli
Mar 30, 2001·Advances in Dental Research·A Mombelli
May 31, 2002·Journal of Clinical Microbiology·B J PasterF E Dewhirst
Dec 23, 2003·Environmental Microbiology·Yair Shemesh, E Jurkevitch
Jul 21, 2004·Nature Reviews. Microbiology·R Elizabeth Sockett, Carey Lambert
Oct 13, 2005·International Endodontic Journal·L BergmansP Lambrechts

Citations

Jan 11, 2011·Molecular Oral Microbiology·A Dashiff, Daniel E Kadouri
Oct 19, 2010·Journal of Bacteriology·Alan J Wolfe
Feb 21, 2014·Journal of Indian Society of Periodontology·K HariniShruthi Hegde
Aug 18, 2010·Periodontology 2000·Brian HendersonDerren Ready
Mar 2, 2016·Environmental Microbiology Reports·Ryan M ChanyiJoanna S Brooke
Mar 5, 2019·Journal of Periodontology·Pedro H F SilvaMichel R Messora
Mar 1, 2012·BMB Reports·Mohammed DwidarRobert J Mitchell
Jan 11, 2011·Molecular Oral Microbiology·M Van EsscheW Teughels
Oct 10, 2020·Computational and Structural Biotechnology Journal·Juana PérezJosé Muñoz-Dorado

Methods Mentioned

BETA
PCR
scanning electron microscopy

Related Concepts

Interference, Bacterial
Bdellovibrio
Streak Plate Count
Aggregatibacter actinomycetemcomitans
Microbial Biofilms
Antibiotics
Gram-Negative Bacteria
Scanning Electron Microscopy
Periodontitis
Plankton

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.