Bdellovibrio bacteriovorus HD100, a predator of Gram-negative bacteria, benefits energetically from Staphylococcus aureus biofilms without predation

The ISME Journal
Hansol ImRobert J Mitchell

Abstract

Bdellovibrio bacteriovorus HD100 is a predatory bacterium which lives by invading the periplasm of Gram-negative bacteria and consuming them from within. Although B. bacteriovorus HD100 attacks only Gram-negative bacterial strains, our work here shows attack-phase predatory cells also benefit from interacting with Gram-positive biofilms. Using Staphylococcus aureus biofilms, we show this predator degrades the biofilm matrix, obtains nutrients and uses these to produce and secrete proteolytic enzymes to continue this process. When exposed to S. aureus biofilms, the transcriptome of B. bacteriovorus HD100 was analogous to that seen when present intraperiplasmically, suggesting it is responding similarly as when in a prey. Moreover, two of the induced proteases (Bd2269 and Bd2692) were purified and their activities against S. aureus biofilms verified. In addition, B. bacteriovorus HD100 gained several clear benefits from its interactions with S. aureus biofilms, including increased ATP pools and improved downstream predatory activities when provided prey.

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Citations

May 12, 2019·Applied and Environmental Microbiology·Łukasz MakowskiJolanta Zakrzewska-Czerwińska
Sep 10, 2019·Environmental Microbiology·Hansol ImRobert J Mitchell
May 1, 2020·Frontiers in Microbiology·Eleni BratanisEwa Bukowska-Faniband
Mar 17, 2020·Applied Microbiology and Biotechnology·Robert J MitchellSeong Yeol Choi
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Kaiyu ZhangYang Wang
Oct 29, 2020·Current Microbiology·K GonzalezMara Cea
Oct 10, 2020·Computational and Structural Biotechnology Journal·Juana PérezJosé Muñoz-Dorado

Methods Mentioned

BETA
protease assay

Related Concepts

Bdellovibrio bacteriovorus
Striadyne
Bacterial Proteins
Peptide Hydrolases
Staphylococcus aureus
Microbial Biofilms
Adenosine Triphosphate
Gram-Negative Bacteria
Gram-Positive Bacteria
Proteolysis

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