Bdellovibrio bacteriovorus inhibits Staphylococcus aureus biofilm formation and invasion into human epithelial cells

Scientific Reports
Ajay Kalanjana MonnappaRobert J Mitchell

Abstract

Bdellovibrio bacteriovorus HD100 is a predatory bacterium that attacks many Gram-negative human pathogens. A serious drawback of this strain, however, is its ineffectiveness against Gram-positive strains, such as the human pathogen Staphylococcus aureus. Here we demonstrate that the extracellular proteases produced by a host-independent B. bacteriovorus (HIB) effectively degrade/inhibit the formation of S. aureus biofilms and reduce its virulence. A 10% addition of HIB supernatant caused a 75% or greater reduction in S. aureus biofilm formation as well as 75% dispersal of pre-formed biofilms. LC-MS-MS analyses identified various B. bacteriovorus proteases within the supernatant, including the serine proteases Bd2269 and Bd2321. Tests with AEBSF confirmed that serine proteases were active in the supernatant and that they impacted S. aureus biofilm formation. The supernatant also possessed a slight DNAse activity. Furthermore, treatment of planktonic S. aureus with the supernatant diminished its ability to invade MCF-10a epithelial cells by 5-fold but did not affect the MCF-10a viability. In conclusion, this study illustrates the hitherto unknown ability of B. bacteriovorus to disperse Gram-positive pathogenic biofilms and mitiga...Continue Reading

References

Feb 13, 1974·Archiv für Mikrobiologie·H M Engelking, R J Seidler
Sep 15, 1996·Hospital Practice·H S Gold, A W Karchmer
Jul 21, 2004·Nature Reviews. Microbiology·R Elizabeth Sockett, Carey Lambert
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul SumbyJames M Musser
Jul 8, 2005·Applied and Environmental Microbiology·Daniel E Kadouri, George A O'Toole
Jul 20, 2006·Journal of Medical Microbiology·Mohammed A Al-Fattani, L Julia Douglas
Jan 16, 2007·Applied Microbiology and Biotechnology·P ChaignonS Jabbouri
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Aug 7, 2007·Journal of Bacteriology·Hans-Curt FlemmingDaniel J Wozniak
Sep 13, 2007·Thrombosis and Haemostasis·D Patric Nitsche-SchmitzGursharan S Chhatwal
Mar 19, 2008·Infection and Immunity·Kati SeidlMarkus Bischoff
Apr 26, 2008·PLoS Pathogens·Blaise R Boles, Alexander R Horswill
Feb 14, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rosina KhanAsad U Khan
Feb 24, 2009·Biomaterials·Evan M HetrickMark H Schoenfisch
Jul 18, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Katherine J LauderdaleAlexander R Horswill
Nov 4, 2010·BMC Microbiology·Svetlin TchatalbachevTrinad Chakraborty
Dec 7, 2010·Trends in Biotechnology·Thomas K WoodQun Ma
Jan 11, 2011·Molecular Oral Microbiology·A Dashiff, Daniel E Kadouri
Mar 1, 2012·BMB Reports·Mohammed DwidarRobert J Mitchell
Mar 29, 2012·Biofouling·C E Marcato-RomainV Dossat-Létisse
Jun 21, 2013·PloS One·Mohammed DwidarRobert J Mitchell

Citations

May 9, 2014·Future Microbiology·Robert M Q Shanks, Daniel E Kadouri
Feb 13, 2016·Proceedings. Biological Sciences·Felix J H HolDaniel A Koster
Sep 26, 2014·Current Opinion in Pharmacology·Christophe BeloinDavid Lebeaux
Nov 20, 2014·PloS One·Ryan M Chanyi, Susan F Koval
Mar 2, 2016·Environmental Microbiology Reports·Ryan M ChanyiJoanna S Brooke
Jun 15, 2016·MicrobiologyOpen·Omotayo Opemipo OyedaraMario Alberto Rodriguez-Perez
Sep 16, 2016·Scientific Reports·Ajay Kalanjana MonnappaRobert J Mitchell
Aug 17, 2016·Scientific Reports·Eric G RomanowskiRobert M Q Shanks
Jun 15, 2017·Médecine sciences : M/S·Edouard Jurkevitch, Stéphan Jacquet
May 29, 2019·Environmental Microbiology·Rajesh SathyamoorthyEdouard Jurkevitch
Sep 14, 2018·International Journal of Molecular Sciences·Taylor R JohnsonDavid M Burmeister
May 1, 2020·Frontiers in Microbiology·Eleni BratanisEwa Bukowska-Faniband
Oct 10, 2020·Computational and Structural Biotechnology Journal·Juana PérezJosé Muñoz-Dorado

Methods Mentioned

BETA
protease assay
electrophoresis
chips
confocal microscopy
Assay

Related Concepts

Epithelial Cells
Gram-Negative Bacteria
Gram-Positive Bacteria
Peptide Hydrolases
Plankton
Staphylococcus aureus
Virulence
4-(2-aminoethyl)benzenesulfonylfluoride
Microbial Biofilms
Bdellovibrio bacteriovorus

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.