BDNF accelerates gene expression in cultured cerebellar granule neurons

Brain Research. Developmental Brain Research
X LinR F Bulleit

Abstract

This study reports that in purified cultures of postnatal cerebellar granule cells, BDNF significantly accelerated GABAA receptor alpha 6 subunit (GABAA alpha 6) mRNA expression, a marker for terminally differentiated cerebellar granule neurons, and also accelerated p21cip1 expression. p21cip1 is a general cyclin-dependent kinase (Cdk) inhibitor that can inhibit progression through the cell cycle. Alternatively, the expression of p27kip1, another Cdk inhibitor closely related to p21cip1, is not modified by BDNF. In cultured granule cells, the increase in p21cip1 expression induced by BDNF occurred after dividing granule cells had left the cell cycle and thus was not required to direct granule neuron precursors out of the cell cycle. p21cip1 may have an alterative function during granule neuron terminal differentiation, separate from its ability to regulate cell cycle exit. This report shows that, in vitro, BDNF accelerates granule cell gene expression and may thus modulate cerebellar granule cell differentiation.

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Citations

Apr 29, 1998·Brain Research. Developmental Brain Research·H Cui, R F Bulleit
Jun 24, 2006·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Dongxiao SunMing D Li
Jun 24, 2006·The Journal of Physiology·Shigetada Nakanishi, Makoto Okazawa
Oct 15, 2003·Neuron·Shin-ichi Ohnuma, William A Harris
Feb 23, 2018·Molecular Biology of the Cell·Baojin DingDaniel L Kilpatrick

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