BDNF-TrkB pathway mediates neuroprotection of hydrogen sulfide against formaldehyde-induced toxicity to PC12 cells

PloS One
Jia-Mei JiangXiao-Qing Tang

Abstract

Formaldehyde (FA) is a common environmental contaminant that has toxic effects on the central nervous system (CNS). Our previous data demonstrated that hydrogen sulfide (H2S), the third endogenous gaseous mediator, has protective effects against FA-induced neurotoxicity. As is known to all, Brain-derived neurotropic factor (BDNF), a member of the neurotrophin gene family, mediates its neuroprotective properties via various intracellular signaling pathways triggered by activating the tyrosine kinase receptor B (TrkB). Intriguingly, our previous data have illustrated the upregulatory role of H2S on BDNF protein expression in the hippocampus of rats. Therefore, in this study, we hypothesized that H2S provides neuroprotection against FA toxicity by regulating BDNF-TrkB pathway. In the present study, we found that NaHS, a donor of H2S, upregulated the level of BDNF protein in PC12 cells, and significantly rescued FA-induced downregulation of BDNF levels. Furthermore, we found that pretreatment of PC12 cells with K252a, an inhibitor of the BDNF receptor TrkB, markedly reversed the inhibition of NaHS on FA-induced cytotoxicity and ablated the protective effects of NaHS on FA-induced oxidative stress, including the accumulation of intr...Continue Reading

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Citations

Oct 2, 2015·Acta Biochimica Et Biophysica Sinica·Shenglan GaoXiaoqing Tang
Oct 30, 2019·Clinical and Experimental Pharmacology & Physiology·Hai-Yao LiuXiao-Qing Tang
Mar 21, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Nahal HabibiJoerg Lahann
Mar 3, 2017·Clinical and Experimental Pharmacology & Physiology·Ying ChenXiao-Qing Tang
Jun 2, 2018·Neural Plasticity·Ulfuara ShefaJunyang Jung
Aug 8, 2021·International Journal of Molecular Sciences·Bartosz PomiernyBogusława Budziszewska

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Protein Assay
MDA
flow cytometry

Software Mentioned

Image J

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