BdorOR88a Modulates the Responsiveness to Methyl Eugenol in Mature Males of Bactrocera dorsalis (Hendel)

Frontiers in Physiology
Huan LiuYongyue Lu

Abstract

Insect attractants are important prevention tools for managing populations of the Oriental fruit fly, Bactrocera dorsalis (Hendel), which is a highly destructive agricultural pest with health implications in tropical and subtropical countries. Methyl eugenol (ME) is still considered the gold standard of B. dorsalis attractants. Mature male flies use their olfactory system to detect ME, but the molecular mechanism underlying their olfactory detection of ME largely remains unclear. Here, we showed that ME activates the odorant receptors OR63a-1 and OR88a in mature B. dorsalis males antennae by RNA-Seq and qRT-PCR analysis. Interestingly, ME only elicited robust responses in the BdorOR88a/BdorOrco-expressing Xenopus oocytes, thus suggesting that BdorOR88a is necessary for ME reception and tropism in B. dorsalis. Next, our indoor behavioral assays demonstrated that BdorOR63a-1 knockdown had no significant effects on ME detection and tropism. By contrast, reducing the BdorOR88a transcript levels led to a significant decrease in the males' responsiveness to ME. Taken together, our results gave novel insight in the understanding of the olfactory background to the Oriental fruit fly's attraction toward ME.

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Citations

May 6, 2021·Insects·Francesca ScolariLucie Vaníčková
Aug 8, 2021·Ecotoxicology and Environmental Safety·Shu-Zhen DengXin-Shuai Wang

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Datasets Mentioned

BETA
KP743726
KP743732
EU621792
AHE38523
SRP124917

Methods Mentioned

BETA
RNA-Seq
electrophoresis
PCR

Software Mentioned

Primer
TGICL Clustering
pCLAMP10
KEGG Automatic Annotation Server
Blast2GO
BLASTx
ClustalW2
house Perl scripts
Trinity
RSEM

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