Beclin1 Binds to Enterovirus 71 3D Protein to Promote the Virus Replication.

Viruses
Qi XiangJianguo Wu

Abstract

Enterovirus 71 (EV71) is the main pathogen causing hand-foot-mouth disease (HFMD) in infants and children, which can also lead to severe neurological diseases and even death. Therefore, understanding the replication mechanism of EV71 is of great significance for the prevention and control of EV71-induced diseases. Beclin1 (BECN1, a mammalian homologue of ATG6 in yeast) is an important core protein for the initiation and the normal process of autophagy in cells. In addition to its involvement in autophagy, Beclin1 has also been reported to play an important role in cancer and innate immune signaling pathways. However, the role of Beclin1 in EV71 replication remains elusive. Here, we primarily found that Beclin1 facilitates EV71 replication in human rhabdomyosarcoma (RD) cells and the autophagy was actually induced, but Beclin1 was not significantly affected at either mRNA level or protein level during early EV71 infection. Further studies discovered that Beclin1 could interacts with EV71 non-structural protein 3D mainly through its evolutionary conserved domain (ECD) and coiled-coiled domain (CCD), thus promoting the replication of EV71 in human rhabdomyosarcoma (RD) cells and human astroglioma (U251) cells. Collectively, we rev...Continue Reading

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Citations

Dec 11, 2020·Cell & Bioscience·Chuanjie ZhangJingfeng Li

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Datasets Mentioned

BETA
JN230523.1

Methods Mentioned

BETA
ubiquitination
Transfection
Assay
Co-Immunoprecipitation
Co-IP
PCR
acetylation

Software Mentioned

Primer Premier
GraphPad Prism

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