Bef medaka mutant reveals the essential role of c-myb in both primitive and definitive hematopoiesis

Developmental Biology
Akemi MoriyamaAkira Kudo

Abstract

Vertebrate hematopoiesis is characterized by two evolutionally conserved phases of development, i.e., primitive hematopoiesis, which is a transient phenomenon in the early embryo, and definitive hematopoiesis, which takes place in the later stages. Beni fuji (bef) was originally isolated as a medaka mutant that has an apparently reduced number of erythrocytes in its peripheral blood. Positional cloning revealed that the bef mutant has a nonsense mutation in the c-myb gene. Previous studies have shown that c-myb is essential for definitive hematopoiesis, and c-myb is now widely used as a marker gene for the onset of definitive hematopoiesis. To analyze the phenotypes of the bef mutant, we performed whole-mount in situ hybridization with gene markers of hematopoietic cells. The bef embryos showed decreased expression of alpha-globin and l-plastin, and a complete loss of mpo1 and rag1 expression, suggesting that the bef embryos had defects not only in erythrocytes but also in other myeloid cells, which indicates that their definitive hematopoiesis was aberrant. Interestingly, we observed a diminution in the number of primitive erythrocytes and a delay in the emergence of primitive macrophages in the bef embryos. These results sugg...Continue Reading

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Citations

Jun 19, 2012·Annual Review of Genomics and Human Genetics·Thomas BoehmIsabell Hess
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cristian Soza-RiedThomas Boehm
Sep 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph S Lipsick
Feb 23, 2013·Biology Open·Colin J DavidsonJoseph S Lipsick
Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Emily Gritz, Karen K Hirschi
Jun 21, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kohei ItoAkira Kudo

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