Behavior of human serum albumin on strong cation exchange resins: I. experimental analysis

Journal of Chromatography. a
Agnes VoitlMassimo Morbidelli

Abstract

Experiments with human serum albumin on the strong cation exchange resin Fractogel EMD SE Hicap (M) were carried out. Even though human serum albumin was used at high purity, two peaks in gradient elution experiments occurred. The obtained data can be explained by considering that human serum albumin binds to Fractogel EMD SE Hicap (M) in two different binding conformations: the protein adsorbs instantaneously in the first conformation and then changes into the second one with a kinetic limitation. The two-peak behavior of human serum albumin was analyzed in detail, especially at various gradient lengths, concentrations and temperatures. Breakthrough curves were performed at four modifier concentrations and three velocities. The characteristic adsorption behavior, found for gradient experiments, was confirmed by the breakthrough curves. The two-peak elution pattern of human serum albumin was also found for other strong cation exchange resins, but not for weak cation exchange resins. It is concluded that the described behavior is peculiar for the interaction of human serum albumin with the strong cation exchange ligand of the resin.

References

Oct 1, 1975·Perspectives in Psychiatric Care·B Putnam
Jul 31, 2007·Journal of Chromatography. a·Arne StabyInge Holm Jensen
Jul 24, 2010·Journal of Chromatography. a·Agnes VoitlMassimo Morbidelli
Aug 13, 2010·Journal of Chromatography. a·Agnes VoitlMassimo Morbidelli

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Citations

Jul 7, 2014·Journal of Chromatography. a·Adrian M GospodarekErik J Fernandez
Jul 10, 2012·Journal of Chromatography. a·Ron GillespieSuresh Vunnum
Sep 30, 2010·Journal of Chromatography. a·Abhijit TarafderMassimo Morbidelli
Jul 24, 2010·Journal of Chromatography. a·Agnes VoitlMassimo Morbidelli
Aug 13, 2010·Journal of Chromatography. a·Agnes VoitlMassimo Morbidelli
Mar 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ohnmar KhanalAbraham M Lenhoff
Jan 17, 2021·Journal of Chromatography. a·Linling Yu, Yan Sun

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