Behavior of interacting species in capillary electrophoresis described by mass transfer equation

Analytical Chemistry
Ning Fang, David D Y Chen

Abstract

Affinity capillary electrophoresis (ACE) has been used to estimate thermodynamic constants of binding interactions with linear or nonlinear regression methods. The accuracy of this approach relies heavily on the binding interaction mechanism, which is controlled by both the nature of the interaction and the experimental conditions. The development of a highly efficient computer-simulated ACE system makes it possible to demonstrate the detailed behavior of any interacting species of a given interaction under any conditions. The order of the mobilities of the complex and the two binding species in their free forms is a key factor to determine what molecules in what locations of the column are involved in the interaction, and the peak shape resulting from such interactions, of a given ACE experiment. In this paper and the supporting materials, 18 scenarios in 6 different combinations of migration orders of the free analyte, free additive, and complex formed are studied by a computer simulation program based on the mass transfer equation. From the study of these situations, we conclude high additive concentration (ensuring high capacity factor) and low analyte concentration (ensuring fast fill-in of the free additive in the analyte...Continue Reading

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Citations

Feb 28, 2009·Electrophoresis·Michael C BreadmoreWolfgang Thormann
Dec 30, 2009·Electrophoresis·Chunxia Jiang, Daniel W Armstrong
Mar 2, 2010·Electrophoresis·Wolfgang ThormannRichard A Mosher
Nov 9, 2019·Electrophoresis·Jitka Caslavska, Wolfgang Thormann
Jul 22, 2021·Electrophoresis·Wolfgang Thormann, Richard A Mosher
Jan 31, 2015·Analytical Chemistry·Thomas M WynneSumita Pennathur

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