Behavior of macrophage and osteoblast cell lines in contact with the β-TCP biomaterial (beta-tricalcium phosphate)

Morphologie : Bulletin De L'Association Des Anatomistes
B Arbez, H Libouban

Abstract

Beta-tricalcium phosphate (β-TCP) is a synthetic ceramic used for filling bone defects. It is a good alternative to autologous grafts since it is biocompatible, resorbable and osteoconductive. Previous in vivo studies have shown that macrophages are one of the first cells coming in contact with the biomaterial followed by osteoclasts and osteoblasts that will elaborate new bone packets. Studies have focused on osteoclast morphology and very few of them have investigated the role of macrophages. The aims of this study were to characterize (i) the biomaterial surface; (ii) the in vitro behavior of macrophages (J774.2 and Raw264.7 cells) using the description of cell morphology by scanning electron microscopy (SEM) at 7 and 14 days; (iii) the behavior of osteoblasts (SaOs-2 and MC3T3-E1 cells) seeded at the surface of the biomaterial 24, 48 and 72hours by SEM and confocal microscopy. Cell proliferation was analyzed by MTT assays. Viability and affinity of the macrophages for β-TCP were found significantly increased after 7 and 14d. MC3T3-E1 cells were anchored and stretched onto the β-TCP surface as early as 24h with a high proliferation rate (+190%) when compared to the surface of a well plate. SaOs-2 exhibited the same morpholog...Continue Reading

Citations

May 10, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tomokazu FurihataTsukasa Akasaka
Jun 11, 2020·Morphologie : Bulletin De L'Association Des Anatomistes·B Arbez, H Libouban
Mar 9, 2020·Micron : the International Research and Review Journal for Microscopy·Daniel ChappardBernard Guillaume
Aug 16, 2019·Micron : the International Research and Review Journal for Microscopy·Baptiste ArbezDaniel Chappard
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mattia BiesuzVincenzo M Sglavo

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