Behavior of peptides and computer-assisted optimization of peptides separations in a normal-phase thin-layer chromatography system with and without the addition of ionic liquid in the eluent

Biomedical Chromatography : BMC
Tomasz BaczekPaulina Juszczyk

Abstract

The addition of an ionic liquid into the mobile phase appeared to be useful in optimization of chromatographic separation of peptides. Different behavior of peptides in thin-layer chromatography (TLC) was observed after addition of 1-ethyl-3-methylimidazolium tetra fluoroborate to the eluent in comparison to the system without the ionic liquid. Nonlinear dependence of the retention coefficient, R(M), of peptides on the volume percentage of acetonitrile in the eluent was found in normal-phase TLC with and without immidazolium tetra fluoroborate in the mobile phase. In general, R(M) increased with increasing concentration of acetonitrile. In TLC systems without the ionic liquid, R(M) can be described well with a quadratic function. On the other hand, in a TLC system with an ionic liquid as the additive to the mobile phase, the retention behavior is better described with a third-degree polynomial function. The potential usefulness of ionic liquids for optimization of separation of peptides was demonstrated. Optimization of the separation conditions was supported by a commercially available computer program.

References

Feb 3, 2000·Analytical Chemistry·J T Mehl, D M Hercules
Jun 27, 2003·Analytical and Bioanalytical Chemistry·Z B AlfassiP Neta

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Citations

May 18, 2013·Analytical and Bioanalytical Chemistry·Leticia B EscuderoRodolfo G Wuilloud
Mar 6, 2016·Journal of Chromatography. a·Radosław Ł GwardaTadeusz H Dzido
Aug 11, 2011·PloS One·Christina M PrestonChristopher A Scholin
Apr 7, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alesia A TietzeDiana Imhof
Jun 6, 2007·Biomedical Chromatography : BMC·T Cserháti
Dec 5, 2008·Chemical Reviews·Jean-Christophe PlaqueventAnnie-Claude Gaumont
Jun 15, 2006·Analytical Chemistry·Joseph Sherma

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