Behavior of supercoiled DNA

Biophysical Journal
T R StrickV Croquette

Abstract

We study DNA supercoiling in a quantitative fashion by micromanipulating single linear DNA molecules with a magnetic field gradient. By anchoring one end of the DNA to multiple sites on a magnetic bead and the other end to multiple sites on a glass surface, we were able to exert torsional control on the DNA. A rotating magnetic field was used to induce rotation of the magnetic bead, and reversibly over- and underwind the molecule. The magnetic field was also used to increase or decrease the stretching force exerted by the magnetic bead on the DNA. The molecule's degree of supercoiling could therefore be quantitatively controlled and monitored, and tethered-particle motion analysis allowed us to measure the stretching force acting on the DNA. Experimental results indicate that this is a very powerful technique for measuring forces at the picoscale. We studied the effect of stretching forces ranging from 0.01 pN to 100 pN on supercoiled DNA (-0.1 < sigma < 0.2) in a variety of ionic conditions. Other effects, such as stretching-relaxing hysteresis and the braiding of two DNA molecules, are discussed.

References

Nov 1, 1977·Journal of Molecular Biology·C M RaddingR C Wiegand
Oct 20, 1992·Journal of Molecular Biology·A V VologodskiiN R Cozzarelli
Feb 20, 1992·Journal of Molecular Biology·T Schlick, W K Olson
Apr 1, 1992·Mikrobiyoloji bülteni·T Koçoğlu, T Yalçinkaya
Jun 5, 1992·Journal of Molecular Biology·C J Benham
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Jun 20, 1990·Journal of Molecular Biology·T C BolesN R Cozzarelli
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D KowalskiM J Eddy
May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J VinogradP Laipis
Sep 30, 1994·Science·A BensimonD Bensimon
Sep 1, 1995·Journal of Molecular Biology·Y Timsit, D Moras
Jul 22, 1994·Science·J F Marko, E D Siggia
Sep 9, 1994·Science·C BustamanteS Smith
Feb 25, 1993·Nature·M Dunaway, E A Ostrander
Mar 29, 1996·Science·T R StrickV Croquette
Feb 9, 1996·Science·P CluzelF Caron
Jul 1, 1996·Biophysical Journal·G JanninkJ L Sikorav
Jan 1, 1996·Annual Review of Biochemistry·J C Wang
Aug 29, 1997·Journal of Molecular Biology·Z Wang, P Dröge
Oct 23, 1997·Biophysical Journal·J F AllemandV Croquette
Sep 1, 1995·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·J F Marko, E D Siggia
Feb 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·L Pauling, R B Corey

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Citations

Aug 27, 1998·Electrophoresis·G W SlaterG Drouin
Jul 19, 2002·Biophysical Journal·Svetlana S ZakharovaJohan R C van der Maarel
May 8, 1999·Biophysical Journal·R RohsR Lavery
Jul 24, 2003·Journal of Molecular Biology·Jordanka Zlatanova, Sanford H Leuba
Nov 15, 2003·Journal of Theoretical Biology·Dalila Laoudj-ChenivesseEmmanuel Tronel-Peyroz
Dec 7, 2000·Progress in Biophysics and Molecular Biology·T StrickD Bensimon
Feb 27, 1999·Current Opinion in Biotechnology·M D Wang
Jun 14, 2000·Current Opinion in Structural Biology·C BustamanteD Smith
Jul 2, 2003·Current Opinion in Structural Biology·Jean-François AllemandVincent Croquette
Aug 2, 2012·Quarterly Reviews of Biophysics·Jonathan M FoggLynn Zechiedrich
Feb 27, 2007·Nature Methods·Christopher DeufelMichelle D Wang
Apr 9, 2008·Nature Structural & Molecular Biology·Jerod L PtacinCarlos Bustamante
Mar 6, 2007·Nature Structural & Molecular Biology·Marcelo NöllmannNicholas R Cozzarelli
Aug 10, 2004·Nature Structural & Molecular Biology·Ralf SeidelCees Dekker
Apr 12, 2005·Nature Structural & Molecular Biology·Omar A SalehJean-François Allemand
Jun 10, 2009·Physical Chemistry Chemical Physics : PCCP·Maxim Y Sheinin, Michelle D Wang
Mar 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrey RevyakinTerence R Strick
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·John van NoortRemus Thei Dame
Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hua BaiJohn F Marko
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marek SimonMichael G Poirier
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carolina CarrascoFernando Moreno-Herrero
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·T R StrickD Bensimon
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J F AllemandV Croquette
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·P Nelson
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Cui, C Bustamante
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B MaierV Croquette
Mar 3, 1999·Journal of Biomolecular Structure & Dynamics·A Lebrun, R Lavery
Apr 13, 2005·Nucleic Acids Research·Thijn van der HeijdenCees Dekker
Aug 29, 2006·Nucleic Acids Research·Timothée LionnetVincent Croquette
Aug 22, 2007·Nucleic Acids Research·Thijn van der HeijdenCees Dekker
Jun 25, 2008·Nucleic Acids Research·Giuseppe LiaLaura Finzi
Jan 9, 2009·Nucleic Acids Research·Xi-Miao HouPeng-Ye Wang
May 12, 2009·Nucleic Acids Research·Marijn T J van LoenhoutCees Dekker
Jun 1, 2010·Nucleic Acids Research·Katrin GüntherRalf Seidel
Jul 6, 2010·Nucleic Acids Research·Domenico SalernoFrancesco Mantegazza
Jan 27, 2011·Nucleic Acids Research·T RamreddyT R Strick
Aug 15, 2002·Nucleic Acids Research·Michael BoninWolfgang Nellen
Nov 15, 2012·Molecular and Cellular Biology·Payel SenBlaine Bartholomew

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