PMID: 15347987Sep 7, 2004Paper

Behavior of tissue-engineered human cartilage after transplantation into nude mice

Journal of Materials Science. Materials in Medicine
Nicole RotterM Sittinger

Abstract

Cartilage lacks the ability to regenerate structural defects. Therefore, autologous grafting has been used routinely to replace cartilaginous lesions. Because tissue engineering of human cartilage with the help of bioresorbable polymer scaffolds is possible in experimental models, the demand for the clinical application grows. In this study we present an analysis of the behavior of transplants made of human chondrocyte pools, agarose and the resorbable polymer scaffold Ethisorb and a preliminary comparison with transplants made of single patients' cells and Ethisorb but without the additional ingredient agarose. Chondrocytes were isolated from the matrix of human septal cartilage by enzymatic digestion. The pool cells were kept in monolayer culture for 2 weeks, the single patients' cells for 3-4 weeks. Chondrocyte pools were suspended in agarose and seeded into the resorbable polymer scaffold Ethisorb. Single patients' cells were seeded without agarose. All cell-polymer constructs were kept in perfusion culture for 10-14 days and transplanted subcutaneously into thymusaplastic nude mice. Additionally we implanted Ethisorb fleeces embedded in agarose without chondrocytes. After 6, 12 and 24 weeks the animals were sacrificed and ...Continue Reading

Citations

Jun 14, 2012·Tissue Engineering. Part a·Silke SchwarzNicole Rotter
Nov 30, 2012·Journal of Tissue Engineering and Regenerative Medicine·Silke SchwarzNicole Rotter
Nov 19, 2011·Journal of Tissue Engineering and Regenerative Medicine·Adelola OseniAlexander M Seifalian
Sep 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Sanshiro KanazawaKazuto Hoshi
Feb 2, 2013·Clinical and Translational Medicine·Will W Minuth, Lucia Denk
May 19, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marsha S ReutherDeborah Watson
Oct 28, 2011·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Angela A ChangDeborah Watson
Jul 28, 2012·Dental Clinics of North America·Orapin V HorstOphir D Klein

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