Behaviors of the "raman spectroscopic signature of life" in single living fission yeast cells under different nutrient, stress, and atmospheric conditions

Applied Spectroscopy
Yu-San HuangHiro-o Hamaguchi

Abstract

Time- and space-resolved Raman spectra of mitochondria in single living fission yeast cells have been measured under various nutrient, stress, and atmospheric conditions. A focus is placed on the behavior of the Raman band located at 1602 cm(-1), which sensitively reflects the metabolic activity in mitochondria and which has been called by us the "Raman spectroscopic signature of life". Addition of nutrients increases the intensity of this band by approximately 1.5 times, confirming its correlation with the metabolic activity in mitochondria. The spectra of cells cultured under 100% N(2), 100% O(2), and N(2)/O(2) (V(N2):V(O2) congruent with approximately = 4:1) atmospheres have been measured for both (16)O(2) and (18)O(2). Yeast cells have been found to lose their metabolic activity after the culture under 100% N(2) and 100% O(2) atmospheres. Cells cultured under a N(2)/(16)O(2) ((16)O(2) = 20%) atmosphere show strong "Raman spectroscopic signature of life". No (18)O isotopic shift has been found for the wavenumber 1602 cm(-1), indicating that the origin of this signature is neither O(2) nor an O-containing small molecule. Addition of H(2)O(2) causes a quick decrease of the "Raman spectroscopic signature of life", followed by t...Continue Reading

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Citations

Jan 22, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·M HarzJ Popp
Mar 7, 2009·Analytical and Bioanalytical Chemistry·Katherine A Willets
Apr 15, 2010·Journal of Biophotonics·Liang-da Chiu, Hiro-o Hamaguchi
May 4, 2010·Journal of Biophotonics·Franziska SchulteJanina Kneipp
Apr 25, 2012·Journal of Biophotonics·Liang-da ChiuHiro-O Hamaguchi
Mar 4, 2011·The Analyst·Li-Lin TayMahmud Bani-Yaghoub
May 1, 2013·Chembiochem : a European Journal of Chemical Biology·Yu-Fang ChiuShinsuke Shigeto
Jul 22, 2011·Chemical Communications : Chem Comm·Chuan-Keng HuangShinsuke Shigeto
Nov 7, 2012·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Venkatesh Kaliaperumal, Hiro-o Hamaguchi
Jul 16, 2009·The Journal of Physical Chemistry. B·Chikao Onogi, Hiro-O Hamaguchi

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