Behaviour of human mesenchymal stem cells on a polyelectrolyte-modified HEMA hydrogel for silk-based ligament tissue engineering

Journal of Biomaterials Science. Polymer Edition
M BosettiM Cannas

Abstract

The aim of this study was to design a functional bio-engineered material to be used as scaffold for autologous mesenchymal stem cells in ligament tissue engineering. Polyelectrolyte modified HEMA hydrogel (HEMA-co-METAC), applied as coating on silk fibroin fibres, has been formulated in order to take advantage of the biocompatibility of the polyelectrolyte by increasing its mechanical properties with silk fibres. Human bone marrow mesenchymal stem cells behaviour on such reinforced polyelectrolyte has been studied by evaluating cell morphology, cell number, attachment, spreading and proliferation together with collagen matrix production and its mRNA expression. Silk fibroin fibres matrices with HEMA-co-METAC coating exhibited acceptable mechanical behaviour compared to the natural ligament, good human mesenchymal stem cell adhesion and with mRNA expression studies higher levels of collagen types I and III expression when compared to control cells on polystyrene. These data indicate high expression of mRNA for proteins responsible for the functional characteristics of the ligaments and suggest a potential for use of this biomaterial in ligament tissue-engineering applications.

References

Nov 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K IrieH Oda
Jun 1, 1995·Biomaterials·S IannaceL Nicolais
Aug 1, 1994·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·D W JacksonT M Simon
Jan 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M G DunnJ P Zawadsky
Jul 1, 1996·Journal of Biomedical Materials Research·K J MargeviciusK Hanselmann
Aug 1, 1997·Mechanisms of Ageing and Development·M DeieS L Woo
Jan 23, 1999·Annals of Biomedical Engineering·E LangelierF A Auger
Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 5, 1999·Clinical Orthopaedics and Related Research·S L WooJ H Wang
Dec 10, 1999·Tissue Engineering·V S LinK L Sung
Oct 31, 2000·Biomaterials·M F GuidoinR Guidoin
Nov 15, 2000·Journal of Biomedical Materials Research·S SofiaD L Kaplan
Dec 5, 2000·The Journal of the American Academy of Orthopaedic Surgeons·S L WooS D Abramowitch
Nov 6, 2001·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·J KartusJ Karlsson
Jan 5, 2002·World Journal of Surgery·S LalanJ P Vacanti
May 15, 2002·Biomacromolecules·Gisela BerthHelmuth Möhwald
Aug 17, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
Nov 9, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
Sep 27, 2003·Tissue Engineering·James Cho-Hong GohEng-Hin Lee
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Jingsong ChenDavid L Kaplan
Apr 22, 2004·Annals of Biomedical Engineering·Savio L Y WooMary T Gabriel
Mar 25, 2005·Journal of Biomedical Materials Research. Part a·S CristinoG Lisignoli
Jun 9, 2005·The Journal of the American Academy of Orthopaedic Surgeons·Robin V West, Christopher D Harner
Nov 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Simon YoungAntonios G Mikos

❮ Previous
Next ❯

Citations

Sep 14, 2010·Biointerphases·V Sánchez-VaqueroM Manso Silván
Jul 30, 2011·Journal of Tissue Engineering and Regenerative Medicine·Luis M Rodriguez-LorenzoJulio San Román
Nov 28, 2012·Advanced Healthcare Materials·Naresh Kasoju, Utpal Bora
Jun 21, 2019·Scientific Reports·Yolanda Martín-MartínDaniel González-Nieto
Feb 6, 2021·International Journal of Molecular Sciences·Weizhen SunXiubo Zhao
Oct 15, 2010·Biomacromolecules·Xiao HuDavid L Kaplan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.