Behaviour of macroglial cells, as identified by their intermediate filament complement, during optic nerve regeneration of Xenopus tadpole

Glia
E Rungger-BrändleM M Messerli

Abstract

Assessment of glial cell behaviour during optic nerve (ON) regeneration in Xenopus tadpoles is hampered by the lack of classical cellular markers that distinguish different glial cells in mammals. We thus have characterized the intermediate filament (IF) complement of tadpole glial cells and used it to follow the fate of glial cell subsets during the first 10 days after ON crush. Glial cells synthesize a restricted number of cytokeratin (CK) species and vimentin. This pattern remains essentially unchanged during metamorphosis and regeneration. However, vimentin turnover is specifically enhanced after injury. The expression of CKs and vimentin has been followed immunocytochemically in situ and in isolated cells recovered from dissociated ON segments. In the normal nerve, 79% of ramified glial cells express both CK and vimentin, 1% CK and 4% vimentin only, whereas 16% express neither IF protein. We tentatively classified CK expressing cells as mature astrocytes and those without IF proteins as oligodendrocytes. In the regenerating ON, the relative number of oligodendrocytes is decreased, while the astrocytic subset becomes accordingly larger but is decreased by day 10 already in favour of cells expressing vimentin only. Astrocyte...Continue Reading

References

Apr 29, 1977·Cell and Tissue Research·L J Stensaas, E R Feringa
Jan 1, 1992·International Review of Cytology·K Albers, E Fuchs
Jul 1, 1992·Journal of Neurobiology·C A StuermerK Paschke
Sep 1, 1991·Brain Research. Molecular Brain Research·I CohenM Schwartz
Apr 1, 1990·The Journal of Cell Biology·R Eichner, M Kahn
Sep 1, 1990·Differentiation; Research in Biological Diversity·S GiordanoN Schechter
Oct 22, 1991·The Journal of Comparative Neurology·R L Levine
Jan 1, 1991·Glia·M E HattenC A Mason
Apr 1, 1991·The Journal of Cell Biology·P A JanmeyM Schliwa
Jan 1, 1990·Anatomy and Embryology·R M GazeJ S Taylor
Jan 1, 1990·Anatomy and Embryology·J S Taylor, R M Gaze
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J K Franz, W W Franke
Dec 1, 1986·Journal of Neurochemistry·S J DeArmondL F Eng
Jan 1, 1985·Cell and Muscle Motility·D Dahl, A Bignami
Jun 1, 1973·The Journal of Experimental Zoology·R A WallaceM W Sega

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Citations

Nov 19, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jie SongDun-Jin Chen
Jun 14, 2013·Genesis : the Journal of Genetics and Development·Dasfne Lee-LiuJuan Larraín

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