Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms

Journal of Neuroendocrinology
Y SchoenfelderUlrich Schmitt

Abstract

P-glycoprotein (P-gp), an efflux transporter localised in the blood-brain barrier, limits the access of multiple xenobiotics to the central nervous system. Whether it is also implemented in the transport of the endogenous glucocorticoid corticosterone is a matter of debate. The P-gp knockout mouse model [abcb1a/b (-/-)] has been shown to differ in the functioning of the hypothalamic-pituitary adrenal (HPA) axis. In the present study, we investigated the behaviour of abcb1a/b (-/-) and wild-type mice with respect to stress-related tests and the effects of corticosterone. Behavioural activities were assessed in the open field (OF) test for 4 days, and in the forced swimming test (FST) and tail suspension test (TST) under naïve and stressed conditions. The FST was also conducted after exogenous corticosterone injection (0.25 and 2.5 mg/kg). Moreover, the elevated plus maze test and the RotaRod test (RotaRod Advanced; TSE Systems, Bad Homburg, Germany) were assessed. Brain corticosterone levels were determined by an immunoassay and expression of glucocorticoid receptors by western blot analysis. Abcb1a/1b (-/-) mice showed significantly decreased brain corticosterone levels and elevated glucocorticoid receptor expression. Behaviour...Continue Reading

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Citations

Sep 27, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Florian SchuckKristina Endres
Oct 29, 2013·Pharmacology, Biochemistry, and Behavior·Olivia F O'LearyJohn F Cryan
Jan 26, 2017·Frontiers in Pharmacology·Natalija PopovićMiroljub Popović

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